Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079558853> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2079558853 endingPage "S4" @default.
- W2079558853 startingPage "S4" @default.
- W2079558853 abstract "In nonhuman primates, mineralocorticoid synthesis by the periovulatory follicle modulates ovarian steroidogenesis (1). To assess whether a similar phenomenon occurs during ovarian stimulation for IVF, follicle fluid (FF) steroid levels were correlated with mural granulosa cell (MGC) lipid content as a cholesterol source for steroidogenesis. Prospective cohort study. FF and MGCs were collected from 27 nonobese women (age, 36.2 ± 4.5 yrs; BMI, 22.6 ± 3.0 kg/m2 [mean ± SD]) undergoing ovarian stimulation for IVF. FF aspirated from the 1st follicle/ovary was used for steroid analysis by liquid chromatography-tandem mass spectrometry. MGCs were suspended in 4% paraformaldehyde, treated with lipid fluorescent dye BODIPY® FL C16 and the nuclear marker DAPI, and quantified by confocal microscopy, using ImageJ software. Pearson correlation coefficients correlated FF steroid levels with MGC lipid content. Independent of patient age, BMI or gonadotropin amount administered, FF levels of the mineralocorticoid, 11-deoxycorticosterone (DOC, 113.5 ± 36.4 nM); the glucocorticoid, 11-dexoycortisol (11DOC, 6.6 ± 3.5 nM); and its precursor, 17-hydroxyprogesterone (4.5 ± 2.3 μM), were positively correlated with MGC lipid content (84 ± 43, fluorescent units/sample) (P≤0.05, all steroids), implying 21 hydroxylase activity. Conversely, FF levels of the 11 hydroxylase-dependent steroids, corticosterone (5.6 ± 3.7 nM) and cortisol (163.4 ± 54.9 nM), were unrelated to MGC lipid content. In FF, DOC and 11DOC levels positively predicted progesterone (DOC: R2 0.73, P≤0.0001; 11DOC: R2 0.23, P≤0.01) and estradiol (DOC: R2 0.23, P≤0.01; 11DOC: R2 0.78, P≤0.0001) levels. Other FF steroid levels were unrelated to MGC lipid content. Mineralocorticoid production by MGCs via 21 hydroxylase likely occurs during ovarian stimulation for IVF and may modulate steroidogenesis." @default.
- W2079558853 created "2016-06-24" @default.
- W2079558853 creator A5016135577 @default.
- W2079558853 creator A5025526143 @default.
- W2079558853 creator A5037101339 @default.
- W2079558853 creator A5045552743 @default.
- W2079558853 creator A5050389002 @default.
- W2079558853 creator A5085029122 @default.
- W2079558853 date "2013-09-01" @default.
- W2079558853 modified "2023-10-16" @default.
- W2079558853 title "Mineralocorticoid production by mural granulosa cells (MGCs) as a modulator of steroidogenesis during ovarian stimulation for in vitro fertilization (IVF)" @default.
- W2079558853 doi "https://doi.org/10.1016/j.fertnstert.2013.07.133" @default.
- W2079558853 hasPublicationYear "2013" @default.
- W2079558853 type Work @default.
- W2079558853 sameAs 2079558853 @default.
- W2079558853 citedByCount "0" @default.
- W2079558853 crossrefType "journal-article" @default.
- W2079558853 hasAuthorship W2079558853A5016135577 @default.
- W2079558853 hasAuthorship W2079558853A5025526143 @default.
- W2079558853 hasAuthorship W2079558853A5037101339 @default.
- W2079558853 hasAuthorship W2079558853A5045552743 @default.
- W2079558853 hasAuthorship W2079558853A5050389002 @default.
- W2079558853 hasAuthorship W2079558853A5085029122 @default.
- W2079558853 hasBestOaLocation W20795588531 @default.
- W2079558853 hasConcept C102570824 @default.
- W2079558853 hasConcept C126322002 @default.
- W2079558853 hasConcept C134018914 @default.
- W2079558853 hasConcept C196843134 @default.
- W2079558853 hasConcept C24998067 @default.
- W2079558853 hasConcept C2776069600 @default.
- W2079558853 hasConcept C2777203538 @default.
- W2079558853 hasConcept C2778324911 @default.
- W2079558853 hasConcept C2778525890 @default.
- W2079558853 hasConcept C2780536345 @default.
- W2079558853 hasConcept C512716672 @default.
- W2079558853 hasConcept C71924100 @default.
- W2079558853 hasConcept C86803240 @default.
- W2079558853 hasConcept C95444343 @default.
- W2079558853 hasConceptScore W2079558853C102570824 @default.
- W2079558853 hasConceptScore W2079558853C126322002 @default.
- W2079558853 hasConceptScore W2079558853C134018914 @default.
- W2079558853 hasConceptScore W2079558853C196843134 @default.
- W2079558853 hasConceptScore W2079558853C24998067 @default.
- W2079558853 hasConceptScore W2079558853C2776069600 @default.
- W2079558853 hasConceptScore W2079558853C2777203538 @default.
- W2079558853 hasConceptScore W2079558853C2778324911 @default.
- W2079558853 hasConceptScore W2079558853C2778525890 @default.
- W2079558853 hasConceptScore W2079558853C2780536345 @default.
- W2079558853 hasConceptScore W2079558853C512716672 @default.
- W2079558853 hasConceptScore W2079558853C71924100 @default.
- W2079558853 hasConceptScore W2079558853C86803240 @default.
- W2079558853 hasConceptScore W2079558853C95444343 @default.
- W2079558853 hasIssue "3" @default.
- W2079558853 hasLocation W20795588531 @default.
- W2079558853 hasOpenAccess W2079558853 @default.
- W2079558853 hasPrimaryLocation W20795588531 @default.
- W2079558853 hasRelatedWork W2003569090 @default.
- W2079558853 hasRelatedWork W2017440399 @default.
- W2079558853 hasRelatedWork W2042397276 @default.
- W2079558853 hasRelatedWork W2054536952 @default.
- W2079558853 hasRelatedWork W2063537644 @default.
- W2079558853 hasRelatedWork W2144188844 @default.
- W2079558853 hasRelatedWork W2149573140 @default.
- W2079558853 hasRelatedWork W2170127865 @default.
- W2079558853 hasRelatedWork W2172100709 @default.
- W2079558853 hasRelatedWork W1492882228 @default.
- W2079558853 hasVolume "100" @default.
- W2079558853 isParatext "false" @default.
- W2079558853 isRetracted "false" @default.
- W2079558853 magId "2079558853" @default.
- W2079558853 workType "article" @default.