Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079560003> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2079560003 endingPage "27" @default.
- W2079560003 startingPage "24" @default.
- W2079560003 abstract "Varicella zoster virus (VZV) PCR is highly sensitive compared to traditional detection methods like culture and direct fluorescent antibody testing (DFA); however, the high cost of commercial assays prohibits their use in many clinical laboratories. Major contributors to cost are the nucleic acid extraction and the PCR reagents. This study evaluated an in-house qualitative real-time PCR where the nucleic acid extraction was replaced by a crude extraction, homogenization and heat treatment. Three methods were compared: virus culture and DFA and real-time PCR following each extraction methods. The real-time PCR was highly specific for VZV, and the analytical sensitivity was equivalent following both extraction methods. In contrast, virus culture and DFA was approximately 10,000-fold less sensitive. Using 200 clinical specimens, the sensitivity for the real-time PCR following nucleic acid extraction or homogenization and heat treatment was essentially equivalent at 100% and 97.2%, respectively; whereas, virus culture and DFA was significantly less sensitive at 54.8%. Overall, homogenization and heat treatment combined with a qualitative in-house real-time PCR is a rapid, accurate and cost effective method for the detection of VZV." @default.
- W2079560003 created "2016-06-24" @default.
- W2079560003 creator A5015954920 @default.
- W2079560003 creator A5043759842 @default.
- W2079560003 creator A5046012658 @default.
- W2079560003 creator A5059132651 @default.
- W2079560003 creator A5073812612 @default.
- W2079560003 date "2014-06-01" @default.
- W2079560003 modified "2023-10-16" @default.
- W2079560003 title "Molecular detection of varicella zoster virus while keeping an eye on the budget" @default.
- W2079560003 cites W1544502025 @default.
- W2079560003 cites W1983674127 @default.
- W2079560003 cites W2010460817 @default.
- W2079560003 cites W2012038159 @default.
- W2079560003 cites W2024887232 @default.
- W2079560003 cites W2065190294 @default.
- W2079560003 cites W2147279811 @default.
- W2079560003 cites W2147368201 @default.
- W2079560003 cites W2150290224 @default.
- W2079560003 cites W2155873501 @default.
- W2079560003 cites W4235256988 @default.
- W2079560003 doi "https://doi.org/10.1016/j.jviromet.2014.02.009" @default.
- W2079560003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24607430" @default.
- W2079560003 hasPublicationYear "2014" @default.
- W2079560003 type Work @default.
- W2079560003 sameAs 2079560003 @default.
- W2079560003 citedByCount "12" @default.
- W2079560003 countsByYear W20795600032014 @default.
- W2079560003 countsByYear W20795600032015 @default.
- W2079560003 countsByYear W20795600032016 @default.
- W2079560003 countsByYear W20795600032019 @default.
- W2079560003 countsByYear W20795600032022 @default.
- W2079560003 countsByYear W20795600032023 @default.
- W2079560003 crossrefType "journal-article" @default.
- W2079560003 hasAuthorship W2079560003A5015954920 @default.
- W2079560003 hasAuthorship W2079560003A5043759842 @default.
- W2079560003 hasAuthorship W2079560003A5046012658 @default.
- W2079560003 hasAuthorship W2079560003A5059132651 @default.
- W2079560003 hasAuthorship W2079560003A5073812612 @default.
- W2079560003 hasConcept C104317684 @default.
- W2079560003 hasConcept C130217890 @default.
- W2079560003 hasConcept C159047783 @default.
- W2079560003 hasConcept C18903297 @default.
- W2079560003 hasConcept C24107716 @default.
- W2079560003 hasConcept C2522874641 @default.
- W2079560003 hasConcept C2776159415 @default.
- W2079560003 hasConcept C2778722038 @default.
- W2079560003 hasConcept C48023723 @default.
- W2079560003 hasConcept C49105822 @default.
- W2079560003 hasConcept C55493867 @default.
- W2079560003 hasConcept C78063203 @default.
- W2079560003 hasConcept C86803240 @default.
- W2079560003 hasConceptScore W2079560003C104317684 @default.
- W2079560003 hasConceptScore W2079560003C130217890 @default.
- W2079560003 hasConceptScore W2079560003C159047783 @default.
- W2079560003 hasConceptScore W2079560003C18903297 @default.
- W2079560003 hasConceptScore W2079560003C24107716 @default.
- W2079560003 hasConceptScore W2079560003C2522874641 @default.
- W2079560003 hasConceptScore W2079560003C2776159415 @default.
- W2079560003 hasConceptScore W2079560003C2778722038 @default.
- W2079560003 hasConceptScore W2079560003C48023723 @default.
- W2079560003 hasConceptScore W2079560003C49105822 @default.
- W2079560003 hasConceptScore W2079560003C55493867 @default.
- W2079560003 hasConceptScore W2079560003C78063203 @default.
- W2079560003 hasConceptScore W2079560003C86803240 @default.
- W2079560003 hasLocation W20795600031 @default.
- W2079560003 hasLocation W20795600032 @default.
- W2079560003 hasOpenAccess W2079560003 @default.
- W2079560003 hasPrimaryLocation W20795600031 @default.
- W2079560003 hasRelatedWork W1846954693 @default.
- W2079560003 hasRelatedWork W2038801503 @default.
- W2079560003 hasRelatedWork W2054848430 @default.
- W2079560003 hasRelatedWork W2056420846 @default.
- W2079560003 hasRelatedWork W2070179873 @default.
- W2079560003 hasRelatedWork W2086839033 @default.
- W2079560003 hasRelatedWork W2099027057 @default.
- W2079560003 hasRelatedWork W2153434009 @default.
- W2079560003 hasRelatedWork W2526756168 @default.
- W2079560003 hasRelatedWork W4366991064 @default.
- W2079560003 hasVolume "202" @default.
- W2079560003 isParatext "false" @default.
- W2079560003 isRetracted "false" @default.
- W2079560003 magId "2079560003" @default.
- W2079560003 workType "article" @default.