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- W2079563833 abstract "Exposure of Bacillus subtilis to a shear rate of 1,482/s leads to a rapid loss of cell viability after 10 h of growth. Biochemical and molecular evidences provided below strongly suggest that cell death under high shear results from an apoptosis-like process similar to that described in eukaryotes, with activation of a caspase-3-like protease (C3LP) followed by DNA fragmentation. Shear stress leads to an increase in specific intracellular reactive oxygen species (siROS), possibly through activation of NADH oxidase (NOX). The formation of siROS precedes the activation of C3LP and DNA fragmentation, thus establishing siROS as the molecular link between shear stress and apoptosis-like cell death. A model is proposed in which NOX is viewed as being strategically placed on the plasma membrane of B. subtilis that senses and converts a mechanical force arising from shear stress into a chemical signal leading to activation of C3LP, DNA fragmentation, and thus, apoptosis-like cell death. © 2006 Wiley Periodicals, Inc." @default.
- W2079563833 created "2016-06-24" @default.
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- W2079563833 date "2006-01-01" @default.
- W2079563833 modified "2023-09-26" @default.
- W2079563833 title "Reactive oxygen species induced by shear stress mediate cell death inBacillus subtilis" @default.
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- W2079563833 doi "https://doi.org/10.1002/bit.20835" @default.
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