FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079573668> ?p ?o ?g. }

• W2079573668 endingPage "1018" @default.
• W2079573668 startingPage "1013" @default.
• W2079573668 abstract "Osteoblasts respond to repetitive strain by activating stretch-activated, nonselective cation channels (SA-CAT) and increasing matrix protein production. SA-CAT channels are thought to be responsible for mechano-transduction in osteoblasts, although the molecular identity of the SA-CAT channel has previously been unknown. We have demonstrated that both the UMR-106 osteoblast-like cell line and human osteoblasts in primary culture express the alpha-subunit of the epithelial sodium channel (alpha-ENaC). The ENaC gene product is closely related to a class of proteins that confer touch sensitivity to Caenorhabditis elegans and are referred to as degenerins. A cDNA clone was obtained of the entire coding region of rat alpha-ENaC (alpha-rENaC). Sequence analysis indicated that the osteoblast clone's sequence was identical to that originally cloned from rat colon. The alpha-rENaC cDNA was cloned into an expression plasmid and transfected into LM(TK-) cells, a null cell for SA-CAT activity. Stable transfectants expressed mRNA and the expected 74-kDa protein corresponding to alpha-rENaC. Reconstitution of alpha-rENaC resulted in the expression of a 24.2 +/- 1.0 psec SA-CAT channel (P(Na):P(K) = 1.1 +/- 0.1). The channel is calcium permeable (P(Na):P(Ca) = 1.4 +/- 0.1) and highly selective for cations over anions (P(Na):P(Cl) >> 20). The channel is only active after negative pressure is applied to cell attached patches, cell swelling, or patch excision. These results represent the first heterologous expression of an SA-CAT channel in a mammalian cell system and provide evidence that the ENaC/degenerin family of proteins are capable of mediating both transepithelial sodium transport and are involved in signal transduction by mechano-sensitive cells such as osteoblasts." @default.
• W2079573668 created "2016-06-24" @default.
• W2079573668 creator A5062375872 @default.
• W2079573668 creator A5084433382 @default.
• W2079573668 creator A5089131644 @default.
• W2079573668 date "1997-02-04" @default.
• W2079573668 modified "2023-10-17" @default.
• W2079573668 title "Reconstitution of stretch-activated cation channels by expression of the α-subunit of the epithelial sodium channel cloned from osteoblasts" @default.
• W2079573668 cites W1496313137 @default.
• W2079573668 cites W1506512444 @default.
• W2079573668 cites W1750172340 @default.
• W2079573668 cites W1855551843 @default.
• W2079573668 cites W1887182106 @default.
• W2079573668 cites W1963928878 @default.
• W2079573668 cites W1968333334 @default.
• W2079573668 cites W1979856671 @default.
• W2079573668 cites W1992469700 @default.
• W2079573668 cites W1993985103 @default.
• W2079573668 cites W199399243 @default.
• W2079573668 cites W2007123896 @default.
• W2079573668 cites W2008790997 @default.
• W2079573668 cites W2026825870 @default.
• W2079573668 cites W2035087930 @default.
• W2079573668 cites W2042225435 @default.
• W2079573668 cites W2053331920 @default.
• W2079573668 cites W2057452403 @default.
• W2079573668 cites W2072977328 @default.
• W2079573668 cites W2075338442 @default.
• W2079573668 cites W2082675476 @default.
• W2079573668 cites W2084690463 @default.
• W2079573668 cites W2087499467 @default.
• W2079573668 cites W2088088901 @default.
• W2079573668 cites W2088766374 @default.
• W2079573668 cites W2090117730 @default.
• W2079573668 cites W2092278636 @default.
• W2079573668 cites W2125942201 @default.
• W2079573668 cites W2158352565 @default.
• W2079573668 cites W2224842634 @default.
• W2079573668 cites W2279667892 @default.
• W2079573668 cites W2406414528 @default.
• W2079573668 cites W3024002380 @default.
• W2079573668 doi "https://doi.org/10.1073/pnas.94.3.1013" @default.
• W2079573668 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/19631" @default.
• W2079573668 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9023374" @default.
• W2079573668 hasPublicationYear "1997" @default.
• W2079573668 type Work @default.
• W2079573668 sameAs 2079573668 @default.
• W2079573668 citedByCount "151" @default.
• W2079573668 countsByYear W20795736682012 @default.
• W2079573668 countsByYear W20795736682013 @default.
• W2079573668 countsByYear W20795736682014 @default.
• W2079573668 countsByYear W20795736682015 @default.
• W2079573668 countsByYear W20795736682016 @default.
• W2079573668 countsByYear W20795736682017 @default.
• W2079573668 countsByYear W20795736682019 @default.
• W2079573668 countsByYear W20795736682020 @default.
• W2079573668 countsByYear W20795736682021 @default.
• W2079573668 countsByYear W20795736682022 @default.
• W2079573668 countsByYear W20795736682023 @default.
• W2079573668 crossrefType "journal-article" @default.
• W2079573668 hasAuthorship W2079573668A5062375872 @default.
• W2079573668 hasAuthorship W2079573668A5084433382 @default.
• W2079573668 hasAuthorship W2079573668A5089131644 @default.
• W2079573668 hasBestOaLocation W20795736682 @default.
• W2079573668 hasConcept C104292427 @default.
• W2079573668 hasConcept C104317684 @default.
• W2079573668 hasConcept C153911025 @default.
• W2079573668 hasConcept C178790620 @default.
• W2079573668 hasConcept C185592680 @default.
• W2079573668 hasConcept C187882448 @default.
• W2079573668 hasConcept C50952357 @default.
• W2079573668 hasConcept C537181965 @default.
• W2079573668 hasConcept C54009773 @default.
• W2079573668 hasConcept C54355233 @default.
• W2079573668 hasConcept C55493867 @default.
• W2079573668 hasConcept C6507245 @default.
• W2079573668 hasConcept C81089528 @default.
• W2079573668 hasConcept C81885089 @default.
• W2079573668 hasConcept C83730767 @default.
• W2079573668 hasConcept C86803240 @default.
• W2079573668 hasConcept C95444343 @default.
• W2079573668 hasConceptScore W2079573668C104292427 @default.
• W2079573668 hasConceptScore W2079573668C104317684 @default.
• W2079573668 hasConceptScore W2079573668C153911025 @default.
• W2079573668 hasConceptScore W2079573668C178790620 @default.
• W2079573668 hasConceptScore W2079573668C185592680 @default.
• W2079573668 hasConceptScore W2079573668C187882448 @default.
• W2079573668 hasConceptScore W2079573668C50952357 @default.
• W2079573668 hasConceptScore W2079573668C537181965 @default.
• W2079573668 hasConceptScore W2079573668C54009773 @default.
• W2079573668 hasConceptScore W2079573668C54355233 @default.
• W2079573668 hasConceptScore W2079573668C55493867 @default.
• W2079573668 hasConceptScore W2079573668C6507245 @default.
• W2079573668 hasConceptScore W2079573668C81089528 @default.
• W2079573668 hasConceptScore W2079573668C81885089 @default.
• W2079573668 hasConceptScore W2079573668C83730767 @default.
• W2079573668 hasConceptScore W2079573668C86803240 @default.
• W2079573668 hasConceptScore W2079573668C95444343 @default.

• next