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- W2079589605 abstract "Chitinase and N-acetylglucosaminidase activities in forest soils were examined by a highly sensitive method using 4-methylumbelliferyl (4MU) derivatives of N-acetyl-D-glucosamine oligomers as substrates. The method involves the fluorometric estimation of the 4-MU released through the activity of the soil enzymes when a soil sample and the substrate are incubated in a buffer. The activities of both enzymes decreased markedly with depth in the 4 forest soil profiles studied (i.e. Andosol, Podzol, 2 Cambisols). The activities of these soil enzymes were highly correlated with the carbon and nitrogen content of the soils. Analyse der Aktivität von Chitinase und N-Acetylglucosaminidase in Waldböden mit Hilfe von 4-Methylumbelliferon-Derivaten Die Aktivität von Chitinase und N-Acetylglucosaminidase in Waldböden wurde mit Hilfe einer sehr empfindlichen Meßmethode, die 4-Methyumbelliferon (4-MU)-Derivate von N-Acetylglucosamin-Oligomeren als Substrat ausnutzt, untersucht. Die Methode besteht u.a. aus einer fluorometrischen Schätzung der Menge des 4-MU, das, nach Inkubation von Bodenproben und Oligomeren in einem Puffer, gebildet wird. Es wurden Profile eines Andosol, eines Podsol, und zweier Cambisole untersucht. Die Aktivität beider Enzyme sank mit der Bodentiefe. Die Enzymaktivität war eng mit den C- und N-Gehalten der Böden korreliert." @default.
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- W2079589605 date "1991-01-01" @default.
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- W2079589605 title "Assay of chitinase and N-acetylglucosaminidase activity in forest soils with 4-methylumbelliferyl derivatives" @default.
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- W2079589605 doi "https://doi.org/10.1002/jpln.19911540304" @default.
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