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• W2079593460 abstract "Transendothelial migration is a crucial step in the complex process of lymphocyte extravasation during lymphocyte homing, immunosurveillance and inflammation. However, little is known about the precise role of cell adhesion molecules (CAM) involved in this particular event. To define the CAM involved in T cell adhesion versus transendothelial migration, we have previously established an in vitro transendothelial migration system using mouse T cells and mouse endothelioma cells. We demonstrate here that, using ICAM-1-deficient endothelioma cells derived from ICAM-1 mutant mice, transendothelial migration of T cells was inhibited to a much greater extent when compared to migration across wild-type cells treated with a blocking anti-ICAM-1 monoclonal antibody. This unexpected result was confirmed by a rescue experiment using retroviral transfer of wild-type ICAM-1 into ICAM-1-deficient endothelial cells. Additional experiments showed that, in the absence of functional ICAM-1, only ICAM-2 was involved in transendothelial migration, but not PECAM-1, VCAM-1, or E-selectin. Taking this novel approach, we show that ICAM-1 and ICAM-2 are essential for transendothelial migration of T cells." @default.
• W2079593460 created "2016-06-24" @default.
• W2079593460 creator A5021769541 @default.
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• W2079593460 date "1998-10-01" @default.
• W2079593460 modified "2023-10-18" @default.
• W2079593460 title "T cell interaction with ICAM-1-deficient endotheliumin vitro: essential role for ICAM-1 and ICAM-2 in transendothelial migration of T cells" @default.
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• W2079593460 doi "https://doi.org/10.1002/(sici)1521-4141(199810)28:10<3086::aid-immu3086>3.0.co;2-z" @default.
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