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• W2079632413 endingPage "469" @default.
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• W2079632413 abstract "Absorption changes coupled with the individual transitions S0–S3 and redox reactions in the water-splitting enzyme system S of photosynthesis have been measured. The principal difficulties of measuring the very small absorption changes in the ultraviolet coupled with those reactions have been reduced drastically through the use of a highly purified Photosystem II complex isolated from the Cyanobacterium synechococcus. The general problem caused by the mixing of the S states during a train of flashes and the falsification through the overlap with absorption changes of QB (binary oscillations) have been treated as follows. (1) The binary oscillations were bypassed through the use of silicomolybdate and high concentrations of DCBQ, respectively, as external electron acceptor. (2) Stable absorption changes of the mixed S-state transitions have been deconvoluted through fitting procedures to get the changes of the individual transitions of S1 → S2 → S3 → S0 → S1. (3) Kinetically resolved absorption changes of the S-states in the 100-μs range gave independent information on the individual transitions. (4) Stable absorption changes of the S0 → S1 transitions in the forefront were induced after shifting the S states through low concentrations of NH2OH two units backwards. Analysis of the resulting sequence Sx → S0 → S1 → S2 → S3 → S0, beginning with an NH2OH depending pre-state, Sx, and followed by an S0 → S1 transition not mixed with the opposite S3 → S0 transition, increased the conclusiveness considerably. It results that the ultraviolet spectrum of the S0 → S1 transition is different from the spectra of the S1 → S2 and S2 → S3 transition. Possible states of manganese, water and surplus charges responsible for these spectra are presented." @default.
• W2079632413 created "2016-06-24" @default.
• W2079632413 creator A5058709494 @default.
• W2079632413 creator A5077187892 @default.
• W2079632413 date "1987-10-01" @default.
• W2079632413 modified "2023-10-01" @default.
• W2079632413 title "Optical characterization of intermediates in the water-splitting enzyme system of photosynthesis — possible states and configurations of manganese and water" @default.
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• W2079632413 doi "https://doi.org/10.1016/0005-2728(87)90097-1" @default.
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