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- W2079634450 abstract "1. The effects of various rabbit sera on the binding of [3H]GABA (4.6 × 10−9M) and on the retention of [14C]sucrose (1.04 × 10−7 M) in a synaptosome-enriched fraction of rat cerebral cortex were examined using differential centrifugation and double-isotope methods. 2. Additions of undiluted whole sera (amounting to about 15th of the total volume of suspensions) decreased the binding of [3H]GABA, did not affect the retention of [14C]sucrose and increased pellet/supernatant [14C]sucrose distribution ratios and protein contents of pellets. 3. Only antiserum to total gangliosides-plus-albumin significantly decreased the binding of [3H]GABA to a greater extent than did control serum; this effect was not due to albumin antibodies since antiserum to albumin did not affect [3H]GABA binding and it was not due to increased protein content of pellets since serum containing hemolyzed erythrocytes (which had a higher protein content than other sera) did not affect [3H]GABA binding. 4. Gangliosides might be involved in modulating the interaction of GABA and its synaptic receptors and/or its transport sites." @default.
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- W2079634450 date "1980-01-01" @default.
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- W2079634450 title "Antiserum to gangliosides inhibits [3H]GABA binding to a synaptosome-enriched fraction of rat cerebral cortex" @default.
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- W2079634450 doi "https://doi.org/10.1016/0306-3623(80)90071-3" @default.
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