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- W2079647221 abstract "The formation of erythrocyte (E)-rosettes by human T-lymphocytes with sheep erythrocytes (SRBC) is the most prevalent method by which T-lymphocytes are quantitated and distinguished from B-lymphocytes. The factors responsible for the binding of SRBC to T-lymphocytes are unknown. The present study was done in order to clarify the physiochemical basis of this cellular interaction, specifically as regards factors that influence the regeneration of SRBC receptors on T-lymphocytes after their removal by trypsin treatment. After exposure of lymphocytes to 0.1% trypsin for 5 minutes, the percentage of rosette forming cells was decreased to less than 10%. After 18 hours incubation at 37° in HBSS, complete regeneration has occurred so that the percentage of rosette forming lymphocytes is not significantly less than values for non-trypsinized cells. Regeneration is inhibited by incubation of lymphocytes with puromycin 20 μg/ml and cyclohexamide 10 μg/ml to levels that are 7% and 33% respectively of values for cells that have regenerated their receptors. The T-lymphocyte receptor for SRBC appears to be a protein structure that is rapidly resynthesized in the absence of serum factors. (Supported by Kiwanis Cancer Research Grant)" @default.
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- W2079647221 date "1977-04-01" @default.
- W2079647221 modified "2023-09-26" @default.
- W2079647221 title "REGENERATION OF T-LYHPHOCYTE RECEPTOR FOR SHEEP ERYTHROCYTES" @default.
- W2079647221 doi "https://doi.org/10.1203/00006450-197704000-00744" @default.
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