FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079653458> ?p ?o ?g. }

• W2079653458 endingPage "586" @default.
• W2079653458 startingPage "576" @default.
• W2079653458 abstract "Malonyl-CoA decarboxylase, a normally mitochondrial enzyme, accumulates in the cytoplasm of specialized glands to cause production of multiple methyl-branched fatty acids. Evidence was presented that a single copy of the decarboxylase gene present in the goose genome codes for both the mitochondrial form found in extremely low amounts in the liver and the cytosolic form found in large amounts in uropygial glands. To elucidate how a single gene encodes both forms, the malonyl-CoA decarboxylase gene and the cDNAs for both the mitochondrial (liver) and the cytoplasmic (gland) species were cloned and sequenced. The decarboxylase gene, found in a 21-kb segment of cloned genomic DNA, is composed of five exons of 0.521, 0.118, 0.156, 0.145, and 1.93 kb interrupted by 6.9, 1.5, 0.45, and 9.3-kb introns. Exon 1 revealed two ATGs in frame 150 bp apart. cDNA for the cytoplasmic form and mitochondrial form showed identical nucleotide sequence, except that the latter was longer than the former. The longest cDNA for the cytoplasmic form of the enzyme extended only 44 bp 5' to the second ATG and the position corresponded to the transcription initiation site of the cytoplasmic form revealed by primer extension and RNase protection. The cDNA for the mitochondrial form isolated from the library extended 19 bp further upstream. Primer extension and RNase protection indicated that transcripts for the mitochondrial form initiated upstream from the first ATG. The N-terminal segment of the open reading frame initiated at the first ATG showed an amphipathic signal sequence appropriate for mitochondrial import. A putative full length mRNA for the mitochondrial form of the enzyme when translated in vitro yielded a 55-kDa primary translation product which was processed by removal of about 5 kDa during uptake into goose liver mitochondria. These results strongly suggest that in most tissues transcription initiates 5'- to the first ATG, generating a transcript that would generate a protein with an N-terminal leader for transport into mitochondria. In the uropygial gland the use of an alternate promoter generates transcripts initiated between the two ATGs and the translation product accumulates in the cytoplasm since it lacks a mitochondrial targeting sequence." @default.
• W2079653458 created "2016-06-24" @default.
• W2079653458 creator A5011765239 @default.
• W2079653458 creator A5025396939 @default.
• W2079653458 creator A5039109230 @default.
• W2079653458 creator A5058723847 @default.
• W2079653458 creator A5060352744 @default.
• W2079653458 creator A5084880003 @default.
• W2079653458 date "1992-11-01" @default.
• W2079653458 modified "2023-10-11" @default.
• W2079653458 title "Cytoplasmic accumulation of a normally mitochondrial malonyl-CoA decarboxylase by the use of an alternate transcription start site" @default.
• W2079653458 cites W1239014656 @default.
• W2079653458 cites W1481258397 @default.
• W2079653458 cites W1526232930 @default.
• W2079653458 cites W1543589576 @default.
• W2079653458 cites W1592169576 @default.
• W2079653458 cites W1598076772 @default.
• W2079653458 cites W1605514660 @default.
• W2079653458 cites W1694661515 @default.
• W2079653458 cites W1781412120 @default.
• W2079653458 cites W1827380001 @default.
• W2079653458 cites W1946332788 @default.
• W2079653458 cites W1972128753 @default.
• W2079653458 cites W1998914312 @default.
• W2079653458 cites W2001199654 @default.
• W2079653458 cites W2012359139 @default.
• W2079653458 cites W2014669590 @default.
• W2079653458 cites W2017144138 @default.
• W2079653458 cites W2021673452 @default.
• W2079653458 cites W2022761661 @default.
• W2079653458 cites W2023354078 @default.
• W2079653458 cites W2038881580 @default.
• W2079653458 cites W2042342410 @default.
• W2079653458 cites W2044196614 @default.
• W2079653458 cites W2045348184 @default.
• W2079653458 cites W2048705954 @default.
• W2079653458 cites W2049661143 @default.
• W2079653458 cites W2052552509 @default.
• W2079653458 cites W2064584733 @default.
• W2079653458 cites W2067595489 @default.
• W2079653458 cites W2071579812 @default.
• W2079653458 cites W2078119356 @default.
• W2079653458 cites W2078983017 @default.
• W2079653458 cites W208059754 @default.
• W2079653458 cites W2085816567 @default.
• W2079653458 cites W2154128645 @default.
• W2079653458 cites W2169922172 @default.
• W2079653458 cites W2224929038 @default.
• W2079653458 cites W4249680347 @default.
• W2079653458 cites W46332365 @default.
• W2079653458 doi "https://doi.org/10.1016/0003-9861(92)90452-3" @default.
• W2079653458 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1416987" @default.
• W2079653458 hasPublicationYear "1992" @default.
• W2079653458 type Work @default.
• W2079653458 sameAs 2079653458 @default.
• W2079653458 citedByCount "29" @default.
• W2079653458 countsByYear W20796534582013 @default.
• W2079653458 countsByYear W20796534582014 @default.
• W2079653458 crossrefType "journal-article" @default.
• W2079653458 hasAuthorship W2079653458A5011765239 @default.
• W2079653458 hasAuthorship W2079653458A5025396939 @default.
• W2079653458 hasAuthorship W2079653458A5039109230 @default.
• W2079653458 hasAuthorship W2079653458A5058723847 @default.
• W2079653458 hasAuthorship W2079653458A5060352744 @default.
• W2079653458 hasAuthorship W2079653458A5084880003 @default.
• W2079653458 hasConcept C104317684 @default.
• W2079653458 hasConcept C10879258 @default.
• W2079653458 hasConcept C153911025 @default.
• W2079653458 hasConcept C167625842 @default.
• W2079653458 hasConcept C187882448 @default.
• W2079653458 hasConcept C194857396 @default.
• W2079653458 hasConcept C24586158 @default.
• W2079653458 hasConcept C36823959 @default.
• W2079653458 hasConcept C47289529 @default.
• W2079653458 hasConcept C54355233 @default.
• W2079653458 hasConcept C67705224 @default.
• W2079653458 hasConcept C86803240 @default.
• W2079653458 hasConceptScore W2079653458C104317684 @default.
• W2079653458 hasConceptScore W2079653458C10879258 @default.
• W2079653458 hasConceptScore W2079653458C153911025 @default.
• W2079653458 hasConceptScore W2079653458C167625842 @default.
• W2079653458 hasConceptScore W2079653458C187882448 @default.
• W2079653458 hasConceptScore W2079653458C194857396 @default.
• W2079653458 hasConceptScore W2079653458C24586158 @default.
• W2079653458 hasConceptScore W2079653458C36823959 @default.
• W2079653458 hasConceptScore W2079653458C47289529 @default.
• W2079653458 hasConceptScore W2079653458C54355233 @default.
• W2079653458 hasConceptScore W2079653458C67705224 @default.
• W2079653458 hasConceptScore W2079653458C86803240 @default.
• W2079653458 hasIssue "2" @default.
• W2079653458 hasLocation W20796534581 @default.
• W2079653458 hasLocation W20796534582 @default.
• W2079653458 hasOpenAccess W2079653458 @default.
• W2079653458 hasPrimaryLocation W20796534581 @default.
• W2079653458 hasRelatedWork W1968861569 @default.
• W2079653458 hasRelatedWork W2093125411 @default.
• W2079653458 hasRelatedWork W2118812285 @default.
• W2079653458 hasRelatedWork W2352701899 @default.

• next