FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079659075> ?p ?o ?g. }

• W2079659075 endingPage "56" @default.
• W2079659075 startingPage "49" @default.
• W2079659075 abstract "Type II diabetes is characterized by deposition of the hormone human Islet Amyloid Polypeptide (hIAPP). Formation of hIAPP amyloid fibrils and aggregates is considered to be responsible for pancreatic β-cell losses. Therefore, insight into the structure of hIAPP in the solid-state and in solution is of fundamental importance in order to better understand the action of small molecules, which can potentially dissolve protein aggregates and modulate cell toxicity. So far, no procedure has been described that allows to obtain the native human IAPP peptide at high yields. We present here a cloning, expression and purification protocol that permits the production of 2.5 and 3 mg of native peptide per liter of minimal and LB medium, respectively. In the construct, hIAPP is fused to a chitin binding domain (CBD). The CBD is subsequently cleaved off making use of intein splicing reaction which yield amidation of the C-terminus. The N-terminus contains a solubilization domain which is cleaved by V8 protease, avoiding additional residues at the N-terminus. The correct formation of the disulfide bond is achieved by oxidation with H2O2." @default.
• W2079659075 created "2016-06-24" @default.
• W2079659075 creator A5009771748 @default.
• W2079659075 creator A5019410078 @default.
• W2079659075 creator A5020044974 @default.
• W2079659075 creator A5040050114 @default.
• W2079659075 creator A5040084490 @default.
• W2079659075 creator A5048483824 @default.
• W2079659075 creator A5075121169 @default.
• W2079659075 date "2015-02-01" @default.
• W2079659075 modified "2023-10-17" @default.
• W2079659075 title "Cloning, expression and purification of the human Islet Amyloid Polypeptide (hIAPP) from Escherichia coli" @default.
• W2079659075 cites W1484278934 @default.
• W2079659075 cites W1557198624 @default.
• W2079659075 cites W1594796409 @default.
• W2079659075 cites W1608388154 @default.
• W2079659075 cites W1964727310 @default.
• W2079659075 cites W1967006902 @default.
• W2079659075 cites W1976110253 @default.
• W2079659075 cites W1977150735 @default.
• W2079659075 cites W1982653993 @default.
• W2079659075 cites W1983360453 @default.
• W2079659075 cites W1983867780 @default.
• W2079659075 cites W1993205343 @default.
• W2079659075 cites W1998834713 @default.
• W2079659075 cites W2017204412 @default.
• W2079659075 cites W2032153132 @default.
• W2079659075 cites W2033820571 @default.
• W2079659075 cites W2038392139 @default.
• W2079659075 cites W2038480642 @default.
• W2079659075 cites W2051518236 @default.
• W2079659075 cites W2055155126 @default.
• W2079659075 cites W2060645826 @default.
• W2079659075 cites W2061245389 @default.
• W2079659075 cites W2067820229 @default.
• W2079659075 cites W2068477603 @default.
• W2079659075 cites W2070360911 @default.
• W2079659075 cites W2079757770 @default.
• W2079659075 cites W2082522919 @default.
• W2079659075 cites W2085632182 @default.
• W2079659075 cites W2091223850 @default.
• W2079659075 cites W2094026322 @default.
• W2079659075 cites W2094190981 @default.
• W2079659075 cites W2097259690 @default.
• W2079659075 cites W2098651156 @default.
• W2079659075 cites W2109939279 @default.
• W2079659075 cites W2113925646 @default.
• W2079659075 cites W2115163996 @default.
• W2079659075 cites W2125747464 @default.
• W2079659075 cites W2128460405 @default.
• W2079659075 cites W2129576519 @default.
• W2079659075 cites W2139111914 @default.
• W2079659075 cites W2140454764 @default.
• W2079659075 cites W2146074741 @default.
• W2079659075 cites W2147844096 @default.
• W2079659075 cites W2328786770 @default.
• W2079659075 doi "https://doi.org/10.1016/j.pep.2014.10.012" @default.
• W2079659075 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25448593" @default.
• W2079659075 hasPublicationYear "2015" @default.
• W2079659075 type Work @default.
• W2079659075 sameAs 2079659075 @default.
• W2079659075 citedByCount "15" @default.
• W2079659075 countsByYear W20796590752015 @default.
• W2079659075 countsByYear W20796590752017 @default.
• W2079659075 countsByYear W20796590752018 @default.
• W2079659075 countsByYear W20796590752020 @default.
• W2079659075 countsByYear W20796590752021 @default.
• W2079659075 countsByYear W20796590752022 @default.
• W2079659075 countsByYear W20796590752023 @default.
• W2079659075 crossrefType "journal-article" @default.
• W2079659075 hasAuthorship W2079659075A5009771748 @default.
• W2079659075 hasAuthorship W2079659075A5019410078 @default.
• W2079659075 hasAuthorship W2079659075A5020044974 @default.
• W2079659075 hasAuthorship W2079659075A5040050114 @default.
• W2079659075 hasAuthorship W2079659075A5040084490 @default.
• W2079659075 hasAuthorship W2079659075A5048483824 @default.
• W2079659075 hasAuthorship W2079659075A5075121169 @default.
• W2079659075 hasConcept C104317684 @default.
• W2079659075 hasConcept C121050878 @default.
• W2079659075 hasConcept C12554922 @default.
• W2079659075 hasConcept C134018914 @default.
• W2079659075 hasConcept C14053116 @default.
• W2079659075 hasConcept C165220095 @default.
• W2079659075 hasConcept C167625842 @default.
• W2079659075 hasConcept C179104552 @default.
• W2079659075 hasConcept C185592680 @default.
• W2079659075 hasConcept C199360897 @default.
• W2079659075 hasConcept C2775954774 @default.
• W2079659075 hasConcept C2777633098 @default.
• W2079659075 hasConcept C2779281246 @default.
• W2079659075 hasConcept C2779306644 @default.
• W2079659075 hasConcept C41008148 @default.
• W2079659075 hasConcept C515207424 @default.
• W2079659075 hasConcept C54458228 @default.
• W2079659075 hasConcept C547475151 @default.
• W2079659075 hasConcept C55493867 @default.
• W2079659075 hasConcept C57711820 @default.
• W2079659075 hasConcept C67705224 @default.

• next