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• W2079661592 abstract "The previously reported streptavidin-TGF alpha chimeric protein-based delivery system (Ohno and Meruelo, DNA Cell Biol. 15:401-406, 1996) could efficiently transfer protein molecules into A431 cells via the epidermal growth factor (EGF) receptor. We have modified this delivery system for the transfer of DNA. For this purpose, we have linked the chimeric protein ST-TGFalpha to DNA through biotinylated polylysine molecules. We show with this system, in the presence of the endosome-destabilizing reagent chloroquine, an average of 50-fold increase in reporter gene expression in comparison with polylysine DNA complexes alone. This gene expression is specific for EGF receptor-expressing cells and is blocked by EGF-binding molecules. These results suggest that the ST-TGF alpha biotinylated polylysine system could be used to deliver DNA to targeted cells." @default.
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• W2079661592 date "1999-10-01" @default.
• W2079661592 modified "2023-10-18" @default.
• W2079661592 title "Targeted Gene Transfer System Using a Streptavidin-Transforming Growth Factor-alpha Chimeric Protein" @default.
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• W2079661592 doi "https://doi.org/10.1089/104454999314881" @default.
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