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• W2079672464 abstract "We have demonstrated that mouse LTA cells synthesize cell-surface heparan sulfate proteoglycans (HSPGs) with regions of defined monosaccharide sequence that specifically interact with antithrombin (HSPGact). It remains unclear how HSPGact can be generated by a biosynthetic pathway with no simple template for directing the ordered assembly of monosaccharide units. To examine this issue, we treated LTA cells with ethyl methanesulfonate and then isolated seven stable mutants that synthesize only 8-27% of the wild-type HSPGact but produce normal amounts of other HSPGs. These mutants are recessive in nature and fall into at least two different complementation groups. The delineation of the molecular basis of these defects should help to elucidate the manner by which cells synthesize HSPGs with regions of defined monosaccharide sequence." @default.
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• W2079672464 date "1990-12-01" @default.
• W2079672464 modified "2023-09-24" @default.
• W2079672464 title "Cell mutants defective in synthesizing a heparan sulfate proteoglycan with regions of defined monosaccharide sequence." @default.
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• W2079672464 doi "https://doi.org/10.1073/pnas.87.24.9784" @default.
• W2079672464 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/55258" @default.
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