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• W2079682685 startingPage "136" @default.
• W2079682685 abstract "In this study, we devised a stationary liquid-phase lab-on-a-chip (SLP LOC), which was operated by moving solid-phase magnetic particles in the stationary liquid phase. The SLP LOC consisted of a sample chamber to which a sample and reactants were added, a detection chamber containing enzyme substrate solution, and a narrow channel connecting the two chambers and filled with buffer. As a model system, competitive immunoassays of saxitoxin (STX), a paralytic shellfish toxin, were conducted in the SLP LOC using protein G-coupled magnetic particles (G-MPs) as the solid phase. Anti-STX antibodies, STX-horseradish peroxidase conjugate, G-MPs, and a STX sample were added to the sample chamber and reacted by shaking. While liquids were in the stationary state, G-MPs were transported from the sample chamber to the detection chamber by moving a magnet below the LOC. After incubation to allow the enzymatic reaction to occur, the absorbance of the detection chamber solution was found to be reciprocally related to the STX concentration of the sample. Thus, the SLP LOC may represent a novel, simple format for point-of-care testing applications of enzyme-linked immunosorbent assays by eliminating complicated liquid handling steps." @default.
• W2079682685 created "2016-06-24" @default.
• W2079682685 creator A5029337135 @default.
• W2079682685 creator A5056447261 @default.
• W2079682685 date "2015-04-01" @default.
• W2079682685 modified "2023-09-25" @default.
• W2079682685 title "Immunoassay of paralytic shellfish toxins by moving magnetic particles in a stationary liquid-phase lab-on-a-chip" @default.
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• W2079682685 doi "https://doi.org/10.1016/j.bios.2014.11.012" @default.
• W2079682685 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25460894" @default.
• W2079682685 hasPublicationYear "2015" @default.
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