Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079683447> ?p ?o ?g. }
- W2079683447 endingPage "1326" @default.
- W2079683447 startingPage "1311" @default.
- W2079683447 abstract "To provide site-specific delivery and targeted release of growth factors to implanted urine-derived stem cells (USCs), we prepared microbeads of alginate containing growth factors. The growth factors included VEGF, IGF-1, FGF-1, PDGF, HGF and NGF. Radiolabeled growth factors were loaded separately and used to access the in vitro release from the microbeads with a gamma counter over 4 weeks. In vitro endothelial differentiation of USCs by the released VEGF from the microbeads in a separate experiment confirmed that the released growth factors from the microbeads were bioactive. USCs and microbeads were mixed with the collagen gel type 1 (2 mg/ml) and used for in vivo studies through subcutaneous injection into nude mice. Four weeks after subcutaneous injection, we found that grafted cell survival was improved and more cells expressed myogenic and endothelial cell transcripts and markers compared to controls. More vessel formation and innervations were observed in USCs combined with six growth factors cocktail incorporated in microbeads compared to controls. In conclusion, a combination of growth factors released locally from the alginate microbeads induced USCs to differentiate into a myogenic lineage, enhanced revascularization and innervation, and stimulated resident cell growth in vivo. This approach could potentially be used for cell therapy in the treatment of stress urinary incontinence." @default.
- W2079683447 created "2016-06-24" @default.
- W2079683447 creator A5000291486 @default.
- W2079683447 creator A5016629990 @default.
- W2079683447 creator A5018005432 @default.
- W2079683447 creator A5036015196 @default.
- W2079683447 creator A5036268550 @default.
- W2079683447 creator A5040883232 @default.
- W2079683447 creator A5047527159 @default.
- W2079683447 creator A5054248284 @default.
- W2079683447 creator A5057287134 @default.
- W2079683447 creator A5069732276 @default.
- W2079683447 creator A5077734839 @default.
- W2079683447 date "2013-01-01" @default.
- W2079683447 modified "2023-10-09" @default.
- W2079683447 title "Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors" @default.
- W2079683447 cites W1512165930 @default.
- W2079683447 cites W1965158070 @default.
- W2079683447 cites W1967689707 @default.
- W2079683447 cites W1973458577 @default.
- W2079683447 cites W1977591373 @default.
- W2079683447 cites W1977598603 @default.
- W2079683447 cites W1987184611 @default.
- W2079683447 cites W1990954463 @default.
- W2079683447 cites W1993532863 @default.
- W2079683447 cites W1995578146 @default.
- W2079683447 cites W2001084378 @default.
- W2079683447 cites W2008925446 @default.
- W2079683447 cites W2011751397 @default.
- W2079683447 cites W2013951106 @default.
- W2079683447 cites W2018042616 @default.
- W2079683447 cites W2019298819 @default.
- W2079683447 cites W2021931341 @default.
- W2079683447 cites W2024024258 @default.
- W2079683447 cites W2026104523 @default.
- W2079683447 cites W2026665804 @default.
- W2079683447 cites W2030806969 @default.
- W2079683447 cites W2036122797 @default.
- W2079683447 cites W2048227994 @default.
- W2079683447 cites W2051752053 @default.
- W2079683447 cites W2057888633 @default.
- W2079683447 cites W2059644490 @default.
- W2079683447 cites W2061940955 @default.
- W2079683447 cites W2064864737 @default.
- W2079683447 cites W2079273552 @default.
- W2079683447 cites W2082092885 @default.
- W2079683447 cites W2082959764 @default.
- W2079683447 cites W2098799479 @default.
- W2079683447 cites W2119532287 @default.
- W2079683447 cites W2119841264 @default.
- W2079683447 cites W2129358933 @default.
- W2079683447 cites W2133759908 @default.
- W2079683447 cites W2153749545 @default.
- W2079683447 cites W2161385880 @default.
- W2079683447 cites W2161908525 @default.
- W2079683447 cites W2190298873 @default.
- W2079683447 cites W4236488639 @default.
- W2079683447 cites W4243298326 @default.
- W2079683447 cites W4361745308 @default.
- W2079683447 cites W4376596785 @default.
- W2079683447 doi "https://doi.org/10.1016/j.biomaterials.2012.10.038" @default.
- W2079683447 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3513922" @default.
- W2079683447 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23137393" @default.
- W2079683447 hasPublicationYear "2013" @default.
- W2079683447 type Work @default.
- W2079683447 sameAs 2079683447 @default.
- W2079683447 citedByCount "96" @default.
- W2079683447 countsByYear W20796834472013 @default.
- W2079683447 countsByYear W20796834472014 @default.
- W2079683447 countsByYear W20796834472015 @default.
- W2079683447 countsByYear W20796834472016 @default.
- W2079683447 countsByYear W20796834472017 @default.
- W2079683447 countsByYear W20796834472018 @default.
- W2079683447 countsByYear W20796834472019 @default.
- W2079683447 countsByYear W20796834472020 @default.
- W2079683447 countsByYear W20796834472021 @default.
- W2079683447 countsByYear W20796834472022 @default.
- W2079683447 countsByYear W20796834472023 @default.
- W2079683447 crossrefType "journal-article" @default.
- W2079683447 hasAuthorship W2079683447A5000291486 @default.
- W2079683447 hasAuthorship W2079683447A5016629990 @default.
- W2079683447 hasAuthorship W2079683447A5018005432 @default.
- W2079683447 hasAuthorship W2079683447A5036015196 @default.
- W2079683447 hasAuthorship W2079683447A5036268550 @default.
- W2079683447 hasAuthorship W2079683447A5040883232 @default.
- W2079683447 hasAuthorship W2079683447A5047527159 @default.
- W2079683447 hasAuthorship W2079683447A5054248284 @default.
- W2079683447 hasAuthorship W2079683447A5057287134 @default.
- W2079683447 hasAuthorship W2079683447A5069732276 @default.
- W2079683447 hasAuthorship W2079683447A5077734839 @default.
- W2079683447 hasBestOaLocation W20796834472 @default.
- W2079683447 hasConcept C136229726 @default.
- W2079683447 hasConcept C1491633281 @default.
- W2079683447 hasConcept C150903083 @default.
- W2079683447 hasConcept C167734588 @default.
- W2079683447 hasConcept C170493617 @default.
- W2079683447 hasConcept C180361614 @default.
- W2079683447 hasConcept C185592680 @default.