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• W2079686505 abstract "Abstract This study offers proteomic elucidation of heat pretreatment‐induced alleviation of UV‐B toxicity in Anabaena doliolum . Heat‐pretreated cells exposed to UV‐B showed improved activity of PSI, PSII, whole chain, 14 C fixation, ATP and NADPH contents compared to UV‐B alone. Proteomic analysis using two‐dimensional gel electrophoresis (2‐DE), MALDI‐TOF MS/MS and reverse transcription polymerase chain reaction (RT‐PCR) of UV‐B and heat pretreatment followed by UV‐B–treated cells exhibited significant and reproducible alterations in nine proteins homologous to phycocyanin‐α‐chain (PC‐α‐chain), phycoerythrocyanin‐α‐chain (PEC‐α‐chain), hypothetical protein alr0882, phycobilisome core component (PBS‐CC), iron superoxide dismutase (Fe‐SOD), fructose‐1,6‐bisphosphate aldolase (FBA), nucleoside diphosphate kinase (NDPK), phosphoribulokinase (PRK) and ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBisCo) large chain. Except the PEC‐α‐chain, hypothetical protein alr0882 and PBS‐CC, all other proteins showed upregulation at low doses of UV‐B (U2) and significant downregulation at higher doses of UV‐B (U5). The disruption of redox status, signaling, pentose phosphate pathway and Calvin cycle appears to be due to the downregulation of Fe‐SOD, NDPK, FBA, PRK and RuBisCo thereby leading to the death of Anabaena . In contrast to this, the upregulation of all the above proteins in heat‐pretreated cells, harboring different heat shock proteins (HSPs) like 60, 26 and 16.6, followed by UV‐B treatment than only the UV‐B–treated ones suggests a protective role of HSPs in mitigating UV‐B toxicity." @default.
• W2079686505 created "2016-06-24" @default.
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• W2079686505 date "2009-04-22" @default.
• W2079686505 modified "2023-10-15" @default.
• W2079686505 title "Heat Pretreatment Alleviates UV-B Toxicity in the Cyanobacterium<i>Anabaena doliolum</i>: A Proteomic Analysis of Cross Tolerance" @default.
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• W2079686505 doi "https://doi.org/10.1111/j.1751-1097.2008.00469.x" @default.
• W2079686505 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19076303" @default.
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