Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079688311> ?p ?o ?g. }
- W2079688311 endingPage "610" @default.
- W2079688311 startingPage "595" @default.
- W2079688311 abstract "During prophase, vertebrate cells disassemble their nuclear envelope (NE) in the process of NE breakdown (NEBD). We have established an in vitro assay that uses mitotic Xenopus laevis egg extracts and semipermeabilized somatic cells bearing a green fluorescent protein-tagged NE marker to study the molecular requirements underlying the dynamic changes of the NE during NEBD by live microscopy. We applied our in vitro system to analyze the role of the Ran guanosine triphosphatase (GTPase) system in NEBD. Our study shows that high levels of RanGTP affect the dynamics of late steps of NEBD in vitro. Also, inhibition of RanGTP production by RanT24N blocks the dynamic rupture of nuclei, suggesting that the local generation of RanGTP around chromatin may serve as a spatial cue in NEBD. Furthermore, the microtubule-depolymerizing drug nocodazole interferes with late steps of nuclear disassembly in vitro. High resolution live cell imaging reveals that microtubules are involved in the completion of NEBD in vivo by facilitating the efficient removal of membranes from chromatin." @default.
- W2079688311 created "2016-06-24" @default.
- W2079688311 creator A5052155088 @default.
- W2079688311 creator A5072646519 @default.
- W2079688311 date "2007-08-13" @default.
- W2079688311 modified "2023-09-29" @default.
- W2079688311 title "An in vitro nuclear disassembly system reveals a role for the RanGTPase system and microtubule-dependent steps in nuclear envelope breakdown" @default.
- W2079688311 cites W1503329918 @default.
- W2079688311 cites W1512975085 @default.
- W2079688311 cites W1531850676 @default.
- W2079688311 cites W1536311055 @default.
- W2079688311 cites W1606409542 @default.
- W2079688311 cites W1629392288 @default.
- W2079688311 cites W1813196431 @default.
- W2079688311 cites W1901442544 @default.
- W2079688311 cites W1953353981 @default.
- W2079688311 cites W1966723276 @default.
- W2079688311 cites W1968620505 @default.
- W2079688311 cites W1971981377 @default.
- W2079688311 cites W1979906352 @default.
- W2079688311 cites W1983077985 @default.
- W2079688311 cites W1983501540 @default.
- W2079688311 cites W1991690400 @default.
- W2079688311 cites W1993222258 @default.
- W2079688311 cites W1996899853 @default.
- W2079688311 cites W1999081109 @default.
- W2079688311 cites W1999319877 @default.
- W2079688311 cites W2001464234 @default.
- W2079688311 cites W2008900071 @default.
- W2079688311 cites W2014294449 @default.
- W2079688311 cites W2017284082 @default.
- W2079688311 cites W2020520526 @default.
- W2079688311 cites W2024547018 @default.
- W2079688311 cites W2033062977 @default.
- W2079688311 cites W2041797033 @default.
- W2079688311 cites W2042192373 @default.
- W2079688311 cites W2042487912 @default.
- W2079688311 cites W2045509241 @default.
- W2079688311 cites W2049469649 @default.
- W2079688311 cites W2055182961 @default.
- W2079688311 cites W2055222724 @default.
- W2079688311 cites W2056204193 @default.
- W2079688311 cites W2058211348 @default.
- W2079688311 cites W2061108475 @default.
- W2079688311 cites W2061152844 @default.
- W2079688311 cites W2061637725 @default.
- W2079688311 cites W2064062498 @default.
- W2079688311 cites W2065634301 @default.
- W2079688311 cites W2065719801 @default.
- W2079688311 cites W2071847651 @default.
- W2079688311 cites W2072274637 @default.
- W2079688311 cites W2073526608 @default.
- W2079688311 cites W2074561346 @default.
- W2079688311 cites W2075488235 @default.
- W2079688311 cites W2080010961 @default.
- W2079688311 cites W2082607775 @default.
- W2079688311 cites W2085805230 @default.
- W2079688311 cites W2095435423 @default.
- W2079688311 cites W2095577003 @default.
- W2079688311 cites W2102550611 @default.
- W2079688311 cites W2103931040 @default.
- W2079688311 cites W2104608706 @default.
- W2079688311 cites W2107139416 @default.
- W2079688311 cites W2107913200 @default.
- W2079688311 cites W2111312305 @default.
- W2079688311 cites W2111939527 @default.
- W2079688311 cites W2119827462 @default.
- W2079688311 cites W2124161758 @default.
- W2079688311 cites W2124427760 @default.
- W2079688311 cites W2138707293 @default.
- W2079688311 cites W2145913988 @default.
- W2079688311 cites W2146563718 @default.
- W2079688311 cites W2158799849 @default.
- W2079688311 cites W2163862712 @default.
- W2079688311 cites W2287660192 @default.
- W2079688311 cites W231956376 @default.
- W2079688311 cites W2403358826 @default.
- W2079688311 cites W2419272096 @default.
- W2079688311 cites W246252021 @default.
- W2079688311 cites W255772229 @default.
- W2079688311 cites W4290260729 @default.
- W2079688311 cites W935855719 @default.
- W2079688311 doi "https://doi.org/10.1083/jcb.200703002" @default.
- W2079688311 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2064467" @default.
- W2079688311 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17698605" @default.
- W2079688311 hasPublicationYear "2007" @default.
- W2079688311 type Work @default.
- W2079688311 sameAs 2079688311 @default.
- W2079688311 citedByCount "88" @default.
- W2079688311 countsByYear W20796883112012 @default.
- W2079688311 countsByYear W20796883112013 @default.
- W2079688311 countsByYear W20796883112014 @default.
- W2079688311 countsByYear W20796883112015 @default.
- W2079688311 countsByYear W20796883112016 @default.
- W2079688311 countsByYear W20796883112017 @default.
- W2079688311 countsByYear W20796883112018 @default.
- W2079688311 countsByYear W20796883112019 @default.
- W2079688311 countsByYear W20796883112020 @default.