Matches in SemOpenAlex for { <https://semopenalex.org/work/W207969810> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W207969810 endingPage "190" @default.
- W207969810 startingPage "181" @default.
- W207969810 abstract "An ultrarapid method for the electrophoretic transfer of high and low molecular weight proteins to nitrocellulose membranes following sodium dodecyl sulfate (SDS) polyacrylamide gel is described here. The transfer was performed with heated (70-75 degrees C) normal transfer buffer from which methanol had been omitted. Complete transfer of high and low molecular weight antigens (molecular weight protein standards, a purified protein, and proteins from a human tissue extract) could be carried out in 10 min for a 7% (0.75 mm) SDS polyacrylamide gel. For 10 and 12.5% gels (0.75 mm) the corresponding time was 15 min. A complete transfer could be carried out in 20 min for 7, 10, and 12.5% gels (1.5 mm gels). The permeability of the gel is increased by heat, such that the proteins trapped in the polyacrylamide gel matrix can be easily transferred to the membrane. The heat mediated transfer method was compared with a conventional transfer protocol, under similar conditions. The conventional method transferred minimal low molecular weight proteins while retaining most of the high molecular weight proteins in the gel. In summary, this procedure is particularly useful for the transfer of high molecular weight proteins, very rapid, and avoids the use of methanol." @default.
- W207969810 created "2016-06-24" @default.
- W207969810 creator A5023848988 @default.
- W207969810 creator A5053902018 @default.
- W207969810 date "2009-01-01" @default.
- W207969810 modified "2023-10-18" @default.
- W207969810 title "Ultrarapid Electrophoretic Transfer of High and Low Molecular Weight Proteins Using Heat" @default.
- W207969810 cites W1979016248 @default.
- W207969810 cites W2002407500 @default.
- W207969810 cites W2011283239 @default.
- W207969810 cites W2044254973 @default.
- W207969810 cites W2055242770 @default.
- W207969810 cites W2063302164 @default.
- W207969810 cites W2086487735 @default.
- W207969810 cites W2100837269 @default.
- W207969810 cites W2101108802 @default.
- W207969810 cites W2163841316 @default.
- W207969810 doi "https://doi.org/10.1007/978-1-59745-542-8_20" @default.
- W207969810 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19378057" @default.
- W207969810 hasPublicationYear "2009" @default.
- W207969810 type Work @default.
- W207969810 sameAs 207969810 @default.
- W207969810 citedByCount "10" @default.
- W207969810 countsByYear W2079698102015 @default.
- W207969810 countsByYear W2079698102016 @default.
- W207969810 countsByYear W2079698102017 @default.
- W207969810 countsByYear W2079698102018 @default.
- W207969810 countsByYear W2079698102021 @default.
- W207969810 crossrefType "book-chapter" @default.
- W207969810 hasAuthorship W207969810A5023848988 @default.
- W207969810 hasAuthorship W207969810A5053902018 @default.
- W207969810 hasConcept C101660505 @default.
- W207969810 hasConcept C116084860 @default.
- W207969810 hasConcept C181199279 @default.
- W207969810 hasConcept C185592680 @default.
- W207969810 hasConcept C188027245 @default.
- W207969810 hasConcept C2778862431 @default.
- W207969810 hasConcept C2778913249 @default.
- W207969810 hasConcept C2778954975 @default.
- W207969810 hasConcept C40250595 @default.
- W207969810 hasConcept C41625074 @default.
- W207969810 hasConcept C43617362 @default.
- W207969810 hasConcept C55493867 @default.
- W207969810 hasConceptScore W207969810C101660505 @default.
- W207969810 hasConceptScore W207969810C116084860 @default.
- W207969810 hasConceptScore W207969810C181199279 @default.
- W207969810 hasConceptScore W207969810C185592680 @default.
- W207969810 hasConceptScore W207969810C188027245 @default.
- W207969810 hasConceptScore W207969810C2778862431 @default.
- W207969810 hasConceptScore W207969810C2778913249 @default.
- W207969810 hasConceptScore W207969810C2778954975 @default.
- W207969810 hasConceptScore W207969810C40250595 @default.
- W207969810 hasConceptScore W207969810C41625074 @default.
- W207969810 hasConceptScore W207969810C43617362 @default.
- W207969810 hasConceptScore W207969810C55493867 @default.
- W207969810 hasLocation W2079698101 @default.
- W207969810 hasLocation W2079698102 @default.
- W207969810 hasOpenAccess W207969810 @default.
- W207969810 hasPrimaryLocation W2079698101 @default.
- W207969810 hasRelatedWork W156659529 @default.
- W207969810 hasRelatedWork W1976518863 @default.
- W207969810 hasRelatedWork W2009541688 @default.
- W207969810 hasRelatedWork W2049881746 @default.
- W207969810 hasRelatedWork W2054151386 @default.
- W207969810 hasRelatedWork W2055088644 @default.
- W207969810 hasRelatedWork W2062386374 @default.
- W207969810 hasRelatedWork W2080343926 @default.
- W207969810 hasRelatedWork W2111817213 @default.
- W207969810 hasRelatedWork W609993229 @default.
- W207969810 isParatext "false" @default.
- W207969810 isRetracted "false" @default.
- W207969810 magId "207969810" @default.
- W207969810 workType "book-chapter" @default.