Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079707727> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2079707727 endingPage "1091" @default.
- W2079707727 startingPage "1081" @default.
- W2079707727 abstract "The synthesis and assembly of spectrin was investigated in erythroid cells during chicken embryo development. Immunoprecipitation of Triton X-100-soluble and -insoluble cytoskeletal fractions with α- and β-spectrin antisera show that, at steady state, α and β spectrin are present in stoichiometric amounts, and exclusively, in the cytoskeleton. However, pulse labeling of cells and in vitro translation of total erythroid cell RNA reveal that α spectrin is synthesized in a two to three fold excess over β spectrin. Pulse-chase experiments show that newly synthesized α and β spectrin are present in both the cytoskeletal and soluble fractions, and that stoichiometric amounts are stably assembled in the cytoskeleton. On the other hand, there is a severalfold excess of α relative to β spectrin in the soluble fraction, both of which turn over with a half-life of 50 min. In cells from 4 day old embryos, more than 80% of the newly synthesized β spectrin, but only 10% of the α spectrin, are present in the cytoskeleton. Thus, early in development, the association of α and β spectrin with the membrane-cytoskeleton may be rate-limited by the amount of β spectrin synthesized. Later on in erythroid development, progressively lesser proportions of newly synthesized β spectrin are present in the cytoskeleton, suggesting that during development, the rate of association of β spectrin with the membrane-cytoskeleton becomes limited by some other membrane-cytoskeletal component." @default.
- W2079707727 created "2016-06-24" @default.
- W2079707727 creator A5001924858 @default.
- W2079707727 creator A5024343507 @default.
- W2079707727 creator A5024528673 @default.
- W2079707727 creator A5069095830 @default.
- W2079707727 date "1983-04-01" @default.
- W2079707727 modified "2023-09-23" @default.
- W2079707727 title "Synthesis and assembly of spectrin during avian erythropoiesis: Stoichiometric assembly but unequal synthesis of α and β spectrin" @default.
- W2079707727 cites W1521213011 @default.
- W2079707727 cites W1529401899 @default.
- W2079707727 cites W1534278553 @default.
- W2079707727 cites W1554943836 @default.
- W2079707727 cites W1605219812 @default.
- W2079707727 cites W1965092010 @default.
- W2079707727 cites W1969188399 @default.
- W2079707727 cites W1969791178 @default.
- W2079707727 cites W1970160164 @default.
- W2079707727 cites W1979067079 @default.
- W2079707727 cites W1983571205 @default.
- W2079707727 cites W2018275392 @default.
- W2079707727 cites W2019675508 @default.
- W2079707727 cites W2032349908 @default.
- W2079707727 cites W2052664734 @default.
- W2079707727 cites W2055121968 @default.
- W2079707727 cites W2059148896 @default.
- W2079707727 cites W2060333964 @default.
- W2079707727 cites W2062051274 @default.
- W2079707727 cites W2063308605 @default.
- W2079707727 cites W2100837269 @default.
- W2079707727 cites W34899889 @default.
- W2079707727 doi "https://doi.org/10.1016/0092-8674(83)90292-1" @default.
- W2079707727 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6220807" @default.
- W2079707727 hasPublicationYear "1983" @default.
- W2079707727 type Work @default.
- W2079707727 sameAs 2079707727 @default.
- W2079707727 citedByCount "111" @default.
- W2079707727 countsByYear W20797077272013 @default.
- W2079707727 countsByYear W20797077272014 @default.
- W2079707727 countsByYear W20797077272015 @default.
- W2079707727 countsByYear W20797077272016 @default.
- W2079707727 countsByYear W20797077272017 @default.
- W2079707727 countsByYear W20797077272018 @default.
- W2079707727 countsByYear W20797077272021 @default.
- W2079707727 countsByYear W20797077272022 @default.
- W2079707727 crossrefType "journal-article" @default.
- W2079707727 hasAuthorship W2079707727A5001924858 @default.
- W2079707727 hasAuthorship W2079707727A5024343507 @default.
- W2079707727 hasAuthorship W2079707727A5024528673 @default.
- W2079707727 hasAuthorship W2079707727A5069095830 @default.
- W2079707727 hasConcept C104317684 @default.
- W2079707727 hasConcept C142669718 @default.
- W2079707727 hasConcept C1491633281 @default.
- W2079707727 hasConcept C153911025 @default.
- W2079707727 hasConcept C50244902 @default.
- W2079707727 hasConcept C55493867 @default.
- W2079707727 hasConcept C71829478 @default.
- W2079707727 hasConcept C74966100 @default.
- W2079707727 hasConcept C86803240 @default.
- W2079707727 hasConcept C95444343 @default.
- W2079707727 hasConceptScore W2079707727C104317684 @default.
- W2079707727 hasConceptScore W2079707727C142669718 @default.
- W2079707727 hasConceptScore W2079707727C1491633281 @default.
- W2079707727 hasConceptScore W2079707727C153911025 @default.
- W2079707727 hasConceptScore W2079707727C50244902 @default.
- W2079707727 hasConceptScore W2079707727C55493867 @default.
- W2079707727 hasConceptScore W2079707727C71829478 @default.
- W2079707727 hasConceptScore W2079707727C74966100 @default.
- W2079707727 hasConceptScore W2079707727C86803240 @default.
- W2079707727 hasConceptScore W2079707727C95444343 @default.
- W2079707727 hasIssue "4" @default.
- W2079707727 hasLocation W20797077271 @default.
- W2079707727 hasLocation W20797077272 @default.
- W2079707727 hasOpenAccess W2079707727 @default.
- W2079707727 hasPrimaryLocation W20797077271 @default.
- W2079707727 hasRelatedWork W1600860784 @default.
- W2079707727 hasRelatedWork W1601094260 @default.
- W2079707727 hasRelatedWork W1971140410 @default.
- W2079707727 hasRelatedWork W1992682995 @default.
- W2079707727 hasRelatedWork W2054013596 @default.
- W2079707727 hasRelatedWork W2076649239 @default.
- W2079707727 hasRelatedWork W2076993927 @default.
- W2079707727 hasRelatedWork W2091067398 @default.
- W2079707727 hasRelatedWork W2409669745 @default.
- W2079707727 hasRelatedWork W2107564180 @default.
- W2079707727 hasVolume "32" @default.
- W2079707727 isParatext "false" @default.
- W2079707727 isRetracted "false" @default.
- W2079707727 magId "2079707727" @default.
- W2079707727 workType "article" @default.