FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079713085> ?p ?o ?g. }

• W2079713085 endingPage "477" @default.
• W2079713085 startingPage "471" @default.
• W2079713085 abstract "Phototransformation of the red-absorbing form of phytochrome (Pr) to the far-red-absorbing form (Pfr) was followed with a custom-built transient spectrum analyzer. Large phytochrome, which consisted of approximately 120000-dalton monomers, was immunopurified or conventionally purified from etiolated oat (Avena sativa L., cv. Garry) shoots. Phototransformation was initiated by exciting Pr with a 115-mJ, 600-ns half-width, 655-nm laser pulse. Absorption spectra were recorded on a microsecond time scale at predetermined times after the flash. It has been reported earlier that flash excitation of large oat Pr produces a transformation intermediate with maximum absorbance near 700 nm in a difference spectrum and that this intermediate decays by two kinetically distinct reactions. Difference spectra for these two reactions are indistinguishable. Both show bleaching centered at 690 nm with no detectable associated absorbance increase between 570 and 830 nm. Subsequent appearance of absorbance at 724 nm, which presumably but not necessarily represents the appearance of Pfr, had earlier been shown to occur by two kinetically distinct reactions for large oat phytochrome. Data presented here indicate in addition the occurrence of a third, slower reaction. Difference spectra for the two faster reactions are indistinguishable, both with maxima near 728 nm and minima near 650 nm. The difference spectrum for the slowest component, however, was qualitatively different exhibiting a maximum near 722 nm with no corresponding minimum. About 15-20% of the absorbance increase at 724 nm occurred by this slowest reaction, which exhibited a half-life of 3 s at 25°C and a Q10 of 1.2 for immunopurified and 1.5 for conventionally purified phytochrome. The percentage occurring by this reaction was independent of temperature over the range studied (1-25dEC). For immunopurified phytochrome the enthalpy of activation, Gibbs free energy of activation, and entropy of activation of this slowest reaction were found to be about lOkJ-mol-1, 75kJ.mol-1, and -220 J.mol-1 K-1, respectively, and for conventionally purified phytochrome 25kJ.mol-1, 75kJ.mol-1and —170 J.mol-1 K-1, respectively. The thermodynamic characteristics of this reaction indicate that it may involve a significant ordering of the protein moiety as it transforms to Pfr." @default.
• W2079713085 created "2016-06-24" @default.
• W2079713085 creator A5025054831 @default.
• W2079713085 creator A5032060149 @default.
• W2079713085 creator A5062606529 @default.
• W2079713085 creator A5081600094 @default.
• W2079713085 date "1982-10-01" @default.
• W2079713085 modified "2023-10-03" @default.
• W2079713085 title "ANALYSIS OF PHOTOTRANSFORMATION INTERMEDIATES IN THE PATHWAY FROM THE RED-ABSORBING TO THE FAR-RED-ABSORBING FORM OF AVENA PHYTOCHROME BY A MULTICHANNEL TRANSIENT SPECTRUM ANALYZER" @default.
• W2079713085 cites W1956168554 @default.
• W2079713085 cites W1966376059 @default.
• W2079713085 cites W1975342582 @default.
• W2079713085 cites W1977967773 @default.
• W2079713085 cites W1981161021 @default.
• W2079713085 cites W2001314239 @default.
• W2079713085 cites W2024247151 @default.
• W2079713085 cites W2070542473 @default.
• W2079713085 cites W2079604554 @default.
• W2079713085 cites W2083700484 @default.
• W2079713085 cites W2084977389 @default.
• W2079713085 cites W2088995206 @default.
• W2079713085 cites W2109606373 @default.
• W2079713085 cites W2110661554 @default.
• W2079713085 cites W2118286461 @default.
• W2079713085 cites W2124166905 @default.
• W2079713085 cites W2155973197 @default.
• W2079713085 cites W2165748907 @default.
• W2079713085 cites W3146257408 @default.
• W2079713085 doi "https://doi.org/10.1111/j.1751-1097.1982.tb04404.x" @default.
• W2079713085 hasPublicationYear "1982" @default.
• W2079713085 type Work @default.
• W2079713085 sameAs 2079713085 @default.
• W2079713085 citedByCount "33" @default.
• W2079713085 crossrefType "journal-article" @default.
• W2079713085 hasAuthorship W2079713085A5025054831 @default.
• W2079713085 hasAuthorship W2079713085A5032060149 @default.
• W2079713085 hasAuthorship W2079713085A5062606529 @default.
• W2079713085 hasAuthorship W2079713085A5081600094 @default.
• W2079713085 hasConcept C113196181 @default.
• W2079713085 hasConcept C119824511 @default.
• W2079713085 hasConcept C120665830 @default.
• W2079713085 hasConcept C121332964 @default.
• W2079713085 hasConcept C1276947 @default.
• W2079713085 hasConcept C185592680 @default.
• W2079713085 hasConcept C2779908699 @default.
• W2079713085 hasConcept C2986477067 @default.
• W2079713085 hasConcept C34742353 @default.
• W2079713085 hasConcept C43617362 @default.
• W2079713085 hasConcept C4839761 @default.
• W2079713085 hasConcept C59822182 @default.
• W2079713085 hasConcept C6929264 @default.
• W2079713085 hasConcept C75473681 @default.
• W2079713085 hasConcept C86803240 @default.
• W2079713085 hasConcept C90180588 @default.
• W2079713085 hasConcept C98015330 @default.
• W2079713085 hasConceptScore W2079713085C113196181 @default.
• W2079713085 hasConceptScore W2079713085C119824511 @default.
• W2079713085 hasConceptScore W2079713085C120665830 @default.
• W2079713085 hasConceptScore W2079713085C121332964 @default.
• W2079713085 hasConceptScore W2079713085C1276947 @default.
• W2079713085 hasConceptScore W2079713085C185592680 @default.
• W2079713085 hasConceptScore W2079713085C2779908699 @default.
• W2079713085 hasConceptScore W2079713085C2986477067 @default.
• W2079713085 hasConceptScore W2079713085C34742353 @default.
• W2079713085 hasConceptScore W2079713085C43617362 @default.
• W2079713085 hasConceptScore W2079713085C4839761 @default.
• W2079713085 hasConceptScore W2079713085C59822182 @default.
• W2079713085 hasConceptScore W2079713085C6929264 @default.
• W2079713085 hasConceptScore W2079713085C75473681 @default.
• W2079713085 hasConceptScore W2079713085C86803240 @default.
• W2079713085 hasConceptScore W2079713085C90180588 @default.
• W2079713085 hasConceptScore W2079713085C98015330 @default.
• W2079713085 hasIssue "4" @default.
• W2079713085 hasLocation W20797130851 @default.
• W2079713085 hasOpenAccess W2079713085 @default.
• W2079713085 hasPrimaryLocation W20797130851 @default.
• W2079713085 hasRelatedWork W1855455844 @default.
• W2079713085 hasRelatedWork W1973211633 @default.
• W2079713085 hasRelatedWork W1981161021 @default.
• W2079713085 hasRelatedWork W2012340821 @default.
• W2079713085 hasRelatedWork W2035765782 @default.
• W2079713085 hasRelatedWork W2058827441 @default.
• W2079713085 hasRelatedWork W2062596385 @default.
• W2079713085 hasRelatedWork W2068645788 @default.
• W2079713085 hasRelatedWork W2073876059 @default.
• W2079713085 hasRelatedWork W2094820178 @default.
• W2079713085 hasVolume "36" @default.
• W2079713085 isParatext "false" @default.
• W2079713085 isRetracted "false" @default.
• W2079713085 magId "2079713085" @default.
• W2079713085 workType "article" @default.