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- W2079717626 abstract "Abstract We describe a novel random amplified polymorphic DNA (RAPD) based technique to rapidly assess the overall success of treatments designed to induce gynogenesis. To test this technique we produced white sturgeon ( Acipenser transmontanus ) meiotic gynogens. A total of 108 putative gynogens of known parentage from four different experimental treatments were screened using RAPD primers which were known to generate sire-specific markers. Only two individuals showed amplification of sire-specific markers indicating that they had received some paternal inheritance and were not true gynogens. This simple RAPD-based technique could be generally applicable for the verification of gynogenesis or androgenesis in other species, especially those which lack suitable phenotypic markers to trace the transmission of parental inheritance. We also determined the ploidy of 2469 diploid, triploid, tetraploid and mosaic sturgeon by using a Coulter Counter to analyze erythrocyte nuclei size and verified the results with flow cytometry. The Coulter Counter proved to be a rapid and accurate technique for ploidy analysis in sturgeon." @default.
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- W2079717626 date "1996-12-01" @default.
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- W2079717626 title "Rapid verification of meiotic gynogenesis and polyploidy in white sturgeon (Acipenser transmontanus Richardson)" @default.
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- W2079717626 doi "https://doi.org/10.1016/s0044-8486(96)01369-5" @default.
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