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- W2079718113 abstract "Clones isolated from the heterogenous Spodoptera frugiperda IPLB-Sf-21-AE and Mamestra brassiace IZD-Mb-O503 insect cell populations have been compared in regards to their synthesis of recombinant Escherichia coli β-galactosidase (β-gal), human tissue plasminogen activator (t-PA), and human kidney renin. Significant differences were found among the clones in their expression levels of these proteins. In addition, the expression levels oft-PA and renin were considerably less than that of β-gal. Clone BC5 was the best producer of t-PA and renin among the Mb-O503 clones, whereas clone BB5 was the best producer of β-gal. The Sf-9 cell line and clone 2 were the best producers of t-PA and renin, respectively, among the Sf-21 clones. The Mb-O503 clones generally were better producers of t-PA than the Sf-21 clones, with the BC5 clone producing twice the levels of any other Mb-O503 or Sf-21 clone. The Sf-21 clones were generally better renin producers; the Mb-O503 clones were generally found to have β-gal expression levels superior to those reported previously for the Sf-21 clones." @default.
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- W2079718113 date "1995-02-01" @default.
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- W2079718113 title "Variation in recombinant protein expression levels among clones of lepidopteran cell populations" @default.
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