Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079719104> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2079719104 endingPage "35" @default.
- W2079719104 startingPage "29" @default.
- W2079719104 abstract "A fluorescence method was used to follow the interaction of 4'-o-tetrahydropyranyladriamycin (THP-ADR) with drug-resistant and -sensitive K562 cells. The amounts of drug bound to the nuclei at the steady state, Cn and at the equilibrium state, CN, once the membrane has been solubilized with Triton X-100, have been determined as a function of the pH outside the cells (pHe): Cn increased and CN decreased as pHe increased. At a given pH value outside the cells, CN is the same for both sensitive and resistant cells, whereas Cn is lower in resistant cells as compared to sensitive cells. Using the observation that the essential binding characteristics of THP-ADR in nuclei are the same for both types of cell, the osmotic active drug concentration, Ci, in the cytoplasm of the cells was determined at different values of pHe. Using fluorescent dye, the cytoplasmic pH was determined and found equal to 7.2 +/- 0.1 in both types of cell. In sensitive cells, the equilibrium transmembrane concentrations verified the relation [DH+]i/[DH+]e = [H +]i/[H+]e where [DH +]i and [DH +]e stand for the concentration of protonated form of the drug inside and outside the cells, respectively. This indicates that the uptake of the drug occurs through free permeation of the neutral form of the drug in response to delta pH gradient. Such a relation is not verified in the case of resistant cells." @default.
- W2079719104 created "2016-06-24" @default.
- W2079719104 creator A5004163998 @default.
- W2079719104 creator A5025825293 @default.
- W2079719104 date "1991-01-01" @default.
- W2079719104 modified "2023-10-16" @default.
- W2079719104 title "Determination of the osmotic active drug concentration in the cytoplasm of anthracycline-resistant and -sensitive K562 cells" @default.
- W2079719104 cites W102012657 @default.
- W2079719104 cites W1509629462 @default.
- W2079719104 cites W1510123979 @default.
- W2079719104 cites W1955333425 @default.
- W2079719104 cites W1963666299 @default.
- W2079719104 cites W1966927466 @default.
- W2079719104 cites W1999652145 @default.
- W2079719104 cites W2006336005 @default.
- W2079719104 cites W2019136523 @default.
- W2079719104 cites W2047351708 @default.
- W2079719104 cites W2048235565 @default.
- W2079719104 cites W2049921340 @default.
- W2079719104 cites W2067037382 @default.
- W2079719104 cites W2069983015 @default.
- W2079719104 cites W2075032348 @default.
- W2079719104 cites W2083610807 @default.
- W2079719104 cites W2119460480 @default.
- W2079719104 cites W2146923243 @default.
- W2079719104 cites W2158952594 @default.
- W2079719104 cites W4247918759 @default.
- W2079719104 doi "https://doi.org/10.1016/0167-4889(91)90217-l" @default.
- W2079719104 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1995065" @default.
- W2079719104 hasPublicationYear "1991" @default.
- W2079719104 type Work @default.
- W2079719104 sameAs 2079719104 @default.
- W2079719104 citedByCount "49" @default.
- W2079719104 countsByYear W20797191042015 @default.
- W2079719104 countsByYear W20797191042018 @default.
- W2079719104 crossrefType "journal-article" @default.
- W2079719104 hasAuthorship W2079719104A5004163998 @default.
- W2079719104 hasAuthorship W2079719104A5025825293 @default.
- W2079719104 hasConcept C121332964 @default.
- W2079719104 hasConcept C12554922 @default.
- W2079719104 hasConcept C1491633281 @default.
- W2079719104 hasConcept C171122931 @default.
- W2079719104 hasConcept C185592680 @default.
- W2079719104 hasConcept C190062978 @default.
- W2079719104 hasConcept C2778461978 @default.
- W2079719104 hasConcept C2780035454 @default.
- W2079719104 hasConcept C2781021840 @default.
- W2079719104 hasConcept C41625074 @default.
- W2079719104 hasConcept C43617362 @default.
- W2079719104 hasConcept C50670333 @default.
- W2079719104 hasConcept C54355233 @default.
- W2079719104 hasConcept C55493867 @default.
- W2079719104 hasConcept C62520636 @default.
- W2079719104 hasConcept C81885089 @default.
- W2079719104 hasConcept C86803240 @default.
- W2079719104 hasConcept C91881484 @default.
- W2079719104 hasConcept C98274493 @default.
- W2079719104 hasConceptScore W2079719104C121332964 @default.
- W2079719104 hasConceptScore W2079719104C12554922 @default.
- W2079719104 hasConceptScore W2079719104C1491633281 @default.
- W2079719104 hasConceptScore W2079719104C171122931 @default.
- W2079719104 hasConceptScore W2079719104C185592680 @default.
- W2079719104 hasConceptScore W2079719104C190062978 @default.
- W2079719104 hasConceptScore W2079719104C2778461978 @default.
- W2079719104 hasConceptScore W2079719104C2780035454 @default.
- W2079719104 hasConceptScore W2079719104C2781021840 @default.
- W2079719104 hasConceptScore W2079719104C41625074 @default.
- W2079719104 hasConceptScore W2079719104C43617362 @default.
- W2079719104 hasConceptScore W2079719104C50670333 @default.
- W2079719104 hasConceptScore W2079719104C54355233 @default.
- W2079719104 hasConceptScore W2079719104C55493867 @default.
- W2079719104 hasConceptScore W2079719104C62520636 @default.
- W2079719104 hasConceptScore W2079719104C81885089 @default.
- W2079719104 hasConceptScore W2079719104C86803240 @default.
- W2079719104 hasConceptScore W2079719104C91881484 @default.
- W2079719104 hasConceptScore W2079719104C98274493 @default.
- W2079719104 hasIssue "1" @default.
- W2079719104 hasLocation W20797191041 @default.
- W2079719104 hasLocation W20797191042 @default.
- W2079719104 hasOpenAccess W2079719104 @default.
- W2079719104 hasPrimaryLocation W20797191041 @default.
- W2079719104 hasRelatedWork W1601437777 @default.
- W2079719104 hasRelatedWork W1978813802 @default.
- W2079719104 hasRelatedWork W2038325347 @default.
- W2079719104 hasRelatedWork W2053153790 @default.
- W2079719104 hasRelatedWork W2071235078 @default.
- W2079719104 hasRelatedWork W2078542920 @default.
- W2079719104 hasRelatedWork W2123520511 @default.
- W2079719104 hasRelatedWork W2359306835 @default.
- W2079719104 hasRelatedWork W2393310753 @default.
- W2079719104 hasRelatedWork W2555571126 @default.
- W2079719104 hasVolume "1091" @default.
- W2079719104 isParatext "false" @default.
- W2079719104 isRetracted "false" @default.
- W2079719104 magId "2079719104" @default.
- W2079719104 workType "article" @default.