FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079723138> ?p ?o ?g. }

• W2079723138 endingPage "202" @default.
• W2079723138 startingPage "197" @default.
• W2079723138 abstract "Ligand blotting procedures were developed for the detection of type 1 plasminogen activator inhibitor (PAI-1) binding protein(s) (BPs) transferred to nitrocellulose sheets after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Purified vitronectin (Vn), a well characterised PAI-1 BP, was employed to optimise this assay system. After blocking with casein, the sheets were washed and then incubated with either 125I-labelled PAI-1 (direct assay), or with unlabelled PAI-1 followed by a polyclonal antiserum to PAI-1 (indirect assay). In the latter case, the bound antibody was detected by using an 125I-labelled second antibody. Binding was dose-dependent with respect to both Vn and PAI-1, and only active PAI-1 bound to Vn (i.e. latent PAI-1 and PAI-1 in complex with tissue-type plasminogen activator (t-PA), did not bind to Vn in this system). Moreover, t-PA, arginine and acidic conditions dissociated PAI-1 from Vn adsorbed to nitrocellulose. Analysis of bovine plasma by these techniques revealed the presence of a single PAI-I BP, and this protein was recognised by antisera to Vn. These results indicate that Vn previously fractionated by SDS-PAGE and transferred to nitrocellulose, continues to bind to PAI-I in a manner that resembles its behaviour in plasma and on extracellular matrix. These ligand blotting procedures may thus represent useful new approaches for the detection of other SDS-stable PAI-1 binding proteins in biological samples." @default.
• W2079723138 created "2016-06-24" @default.
• W2079723138 creator A5012340452 @default.
• W2079723138 creator A5026463840 @default.
• W2079723138 creator A5077803529 @default.
• W2079723138 date "1990-10-01" @default.
• W2079723138 modified "2023-09-23" @default.
• W2079723138 title "Detection of vitronectin by ligand blotting with type 1 plasminogen activator inhibitor" @default.
• W2079723138 cites W1493779021 @default.
• W2079723138 cites W1531765010 @default.
• W2079723138 cites W1550499229 @default.
• W2079723138 cites W1551410107 @default.
• W2079723138 cites W1571398079 @default.
• W2079723138 cites W1572187798 @default.
• W2079723138 cites W1595622217 @default.
• W2079723138 cites W160172706 @default.
• W2079723138 cites W1893939249 @default.
• W2079723138 cites W1983503063 @default.
• W2079723138 cites W2003533505 @default.
• W2079723138 cites W2014316985 @default.
• W2079723138 cites W2027077889 @default.
• W2079723138 cites W2033873544 @default.
• W2079723138 cites W2052818567 @default.
• W2079723138 cites W2100837269 @default.
• W2079723138 cites W2120202594 @default.
• W2079723138 cites W2134311390 @default.
• W2079723138 cites W2146643228 @default.
• W2079723138 cites W23122061 @default.
• W2079723138 cites W2408590235 @default.
• W2079723138 cites W2408781189 @default.
• W2079723138 cites W2410308510 @default.
• W2079723138 cites W258376682 @default.
• W2079723138 cites W4244633958 @default.
• W2079723138 doi "https://doi.org/10.1016/0268-9499(90)90014-b" @default.
• W2079723138 hasPublicationYear "1990" @default.
• W2079723138 type Work @default.
• W2079723138 sameAs 2079723138 @default.
• W2079723138 citedByCount "7" @default.
• W2079723138 crossrefType "journal-article" @default.
• W2079723138 hasAuthorship W2079723138A5012340452 @default.
• W2079723138 hasAuthorship W2079723138A5026463840 @default.
• W2079723138 hasAuthorship W2079723138A5077803529 @default.
• W2079723138 hasConcept C101660505 @default.
• W2079723138 hasConcept C104317684 @default.
• W2079723138 hasConcept C116084860 @default.
• W2079723138 hasConcept C134018914 @default.
• W2079723138 hasConcept C149232098 @default.
• W2079723138 hasConcept C153911025 @default.
• W2079723138 hasConcept C159654299 @default.
• W2079723138 hasConcept C164144092 @default.
• W2079723138 hasConcept C170493617 @default.
• W2079723138 hasConcept C181199279 @default.
• W2079723138 hasConcept C185592680 @default.
• W2079723138 hasConcept C195687474 @default.
• W2079723138 hasConcept C203014093 @default.
• W2079723138 hasConcept C2778913249 @default.
• W2079723138 hasConcept C2778954975 @default.
• W2079723138 hasConcept C2779679481 @default.
• W2079723138 hasConcept C41625074 @default.
• W2079723138 hasConcept C55493867 @default.
• W2079723138 hasConcept C85265743 @default.
• W2079723138 hasConcept C86803240 @default.
• W2079723138 hasConcept C91523082 @default.
• W2079723138 hasConcept C956191 @default.
• W2079723138 hasConceptScore W2079723138C101660505 @default.
• W2079723138 hasConceptScore W2079723138C104317684 @default.
• W2079723138 hasConceptScore W2079723138C116084860 @default.
• W2079723138 hasConceptScore W2079723138C134018914 @default.
• W2079723138 hasConceptScore W2079723138C149232098 @default.
• W2079723138 hasConceptScore W2079723138C153911025 @default.
• W2079723138 hasConceptScore W2079723138C159654299 @default.
• W2079723138 hasConceptScore W2079723138C164144092 @default.
• W2079723138 hasConceptScore W2079723138C170493617 @default.
• W2079723138 hasConceptScore W2079723138C181199279 @default.
• W2079723138 hasConceptScore W2079723138C185592680 @default.
• W2079723138 hasConceptScore W2079723138C195687474 @default.
• W2079723138 hasConceptScore W2079723138C203014093 @default.
• W2079723138 hasConceptScore W2079723138C2778913249 @default.
• W2079723138 hasConceptScore W2079723138C2778954975 @default.
• W2079723138 hasConceptScore W2079723138C2779679481 @default.
• W2079723138 hasConceptScore W2079723138C41625074 @default.
• W2079723138 hasConceptScore W2079723138C55493867 @default.
• W2079723138 hasConceptScore W2079723138C85265743 @default.
• W2079723138 hasConceptScore W2079723138C86803240 @default.
• W2079723138 hasConceptScore W2079723138C91523082 @default.
• W2079723138 hasConceptScore W2079723138C956191 @default.
• W2079723138 hasIssue "4" @default.
• W2079723138 hasLocation W20797231381 @default.
• W2079723138 hasOpenAccess W2079723138 @default.
• W2079723138 hasPrimaryLocation W20797231381 @default.
• W2079723138 hasRelatedWork W1972819401 @default.
• W2079723138 hasRelatedWork W1993627674 @default.
• W2079723138 hasRelatedWork W2064695980 @default.
• W2079723138 hasRelatedWork W2079723138 @default.
• W2079723138 hasRelatedWork W2086630637 @default.
• W2079723138 hasRelatedWork W2104295420 @default.
• W2079723138 hasRelatedWork W2412080718 @default.
• W2079723138 hasRelatedWork W2414178178 @default.

• next