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- W2079729567 abstract "Abstract This paper describes a procedure in which 24-well multidishes and an eight-channel pipette were used in physiological tests to characterize proteolytic, psychorotrophic and, mainly fluorescent, pseudomonads which had been isolated from refrigerated raw milk. Some of these tests have not been duplicated by any of the currently available commercial identification systems. Of the 41 biochemical tests examined, 32 could be performed in multidishes. Multidishes could be used in tests to determine carbon source utilization (27 substrates), growth on MacConkey and cetrimide agars, pigment production on King B agar, gelatin hydrolysis and levan production. Since four tests could be simultaneously inoculated, with less than 2.0 ml of medium per test, the properties of a large number of strains could be rapidly and economically determined. Differences between multidish and conventional test results in the 37°C growth test depended upon whether strains were grown on agar or in liquid medium. Hydrolyses of DNA, butterfat and egg yolk and the dissolution of crystals on tyrosine agar were more easily read when tests were performed in petri dishes than in multidishes. False positive results were obtained for melanin production on tyrosine agar. Arginine dihydrolase, Simmons' citrate and urease tests could not be performed in multidishes since the diffusion of an alkaline product, presumably ammonia, through the headspace above wells resulted in false positive reactions." @default.
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- W2079729567 date "1988-08-01" @default.
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- W2079729567 title "Application of multidishes to the characterization of proteolytic psychrotrophs from raw milk" @default.
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- W2079729567 doi "https://doi.org/10.1016/0167-7012(88)90002-4" @default.
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