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- W2079733715 abstract "Staphylococcus C(Urem Cowan I strain (SAC) is a polyclonal B-cell activator used for in vitro lymphocyte stimulation studies [l]. Its cell wall is rich in protein A, which reacts with the Fc fraction of IgG [2,3], but may also react with Fab fractions [2,4,5], and with IgM [6]. The B-cell stimulation is mediated through protein A interaction with their surface immunoglobulins (Ig) [7], and is T-cell independent [8], although subsequent maturation into Ig-secreting cells is T-cell dependent [8]. SAC in concentrations of 0.0005 to 0.1% has been shown to stimulate lymphocytes in vitro to secrete IgG, IgM, and IgM RF [8-121. The Ig responses in these studies are measured by enzyme-linked immunosorbent assay (ELISA). SAC may potentially interfere with subsequent ELISA readings of Ig concentrations in supernatant of such lymphocyte stimulation assays: Protein A on the SAC cells might bind IgG (and other Ig) secreted by lymphocytes, lowering their concentration in the supernatant. Furthermore, protein A could elute off SAC cells [13] then bind to IgG in the solid phase of the ELISA. This may result in either inhibition of Ig binding causing false negative readings, or binding of protein A to the enzymelabelled Ig, causing false positive readings. To determine whether SAC in concentrations used to stimulate lymphocytes, inteferes significantly with determination of Ig concentration by ELISAs, we have studied in vitro the effects of preincubation of SAC with IgG, IgM, and IgM rheumatoid factor (RF), on the subsequent determination of these Ig by ELISA." @default.
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- W2079733715 date "1990-05-01" @default.
- W2079733715 modified "2023-10-18" @default.
- W2079733715 title "The effect of incubation with Staphylococcus aureus Cowan I on subsequent determinations of IgG, IgM, and IgM rheumatoid factor by ELISA" @default.
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- W2079733715 doi "https://doi.org/10.1016/0009-8981(90)90208-a" @default.
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