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• W2079733929 startingPage "547" @default.
• W2079733929 abstract "Conventional methods for detecting single nucleotide polymorphisms (SNPs), including direct DNA sequencing, pyrosequencing, and melting curve analysis, are to a great extent limited by their requirement for particular detection instruments. To overcome this limitation, we established a novel SNP detection technique utilizing multiple primer extension (MPEX) on a phospholipid polymer-coated surface. This technique is based on the development of a new plastic S-BIO PrimeSurface with a biocompatible polymer; its surface chemistry offers extraordinarily stable thermal properties, as well as chemical properties advantageous for enzymatic reactions on the surface. To visualize allele-specific PCR products on the surface, biotin-dUTP was incorporated into newly synthesized PCR products during the extension reaction. The products were ultimately detected by carrying out a colorimetric reaction with substrate solution containing 4-nitro-blue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP). We demonstrated the significance of this novel SNP detection technique by analyzing representative SNPs on 4 LD blocks of the micro opioid receptor gene. We immobilized 20 allele-specific oligonucleotides on this substrate, and substantially reproduced the results previously obtained by other methods." @default.
• W2079733929 created "2016-06-24" @default.
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• W2079733929 date "2007-01-01" @default.
• W2079733929 modified "2023-09-24" @default.
• W2079733929 title "A novel SNP detection technique utilizing a multiple primer extension (MPEX) on a phospholipid polymer-coated surface" @default.
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• W2079733929 doi "https://doi.org/10.1039/b701645j" @default.
• W2079733929 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17639130" @default.
• W2079733929 hasPublicationYear "2007" @default.
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