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• W2079735511 abstract "DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 84:17-24 (2009) - DOI: https://doi.org/10.3354/dao02024 PCR assay for improved diagnostics of epitheliotropic disease virus (EEDV) in lake trout Salvelinus namaycush T. Kurobe1, S. Marcquenski2, R. P. Hedrick1,* 1Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616, USA 2Wisconsin Department of Natural Resources, Box 7921 Madison, Wisconsin 53707, USA *Corresponding author. Email: rphedrick@ucdavis.edu ABSTRACT: Epizootic epitheliotropic disease virus (EEDV) has caused catastrophic losses among hatchery-reared juvenile lake trout Salvelinus namaycush since the early 1980s and remains a major impediment to lake trout restoration in the Great Lakes basin of the USA. Although EEDV has been tentatively designated as a herpesvirus based upon morphological criteria, further characterization of the virus and development of improved detection methods have been hampered by the inability to propagate the virus in cell culture. Recently obtained sequence data for a region of the putative terminase gene from EEDV as well as the related Salmonid herpesvirus 1 and 2 have permitted the development of a polymerase chain reaction (PCR) assay for specific detection of EEDV. The new EEDV PCR demonstrated both an excellent analytic sensitivity and specificity and detected viral DNA as present in the skin of lake trout during periods of active viral outbreaks. In addition, EEDV DNA was detected among healthy appearing juveniles and in the ovarian fluids of spawning adults. Here we describe the development and initial validation steps of the EEDV PCR as a replacement for current diagnostic methods that require virus extraction from the skin, partial purification by isopycnic centrifugation, and visualization of negatively-stained virions by electron microscopy. KEY WORDS: Fish herpesvirus · Salmonid herpesvirus · Lake trout · EEDV Full text in pdf format PreviousNextCite this article as: Kurobe T, Marcquenski S, Hedrick RP (2009) PCR assay for improved diagnostics of epitheliotropic disease virus (EEDV) in lake trout Salvelinus namaycush. Dis Aquat Org 84:17-24. https://doi.org/10.3354/dao02024Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 84, No. 1. Online publication date: March 09, 2009 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2009 Inter-Research." @default.
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• W2079735511 title "PCR assay for improved diagnostics of epitheliotropic disease virus (EEDV) in lake trout Salvelinus namaycush" @default.
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