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• W2079744699 abstract "Differences between the crystal structures of inhibitor-bound and uninhibited forms of phosphofructokinase (PFK) from B. stearothermophilus have led to a structural model for allosteric inhibition by phosphoenolpyruvate (PEP) wherein a dimer-dimer interface within the tetrameric enzyme undergoes a quaternary shift. We have developed a labeling and hybridization technique to generate a tetramer with subunits simultaneously containing two different extrinsic fluorophores in known subunit orientations. This construct has been utilized in the examination of the effects of allosteric ligand and substrate binding on the subunit affinities of tetrameric PFK using several biophysical and spectroscopic techniques including 2-photon, dual-channel fluorescence correlation spectroscopy (FCS). We demonstrate that PEP-binding at the allosteric site is sufficient to reduce the affinity of the active site interface from beyond the limits of experimental detection to nanomolar affinity, while conversely strengthening the interface at which it is bound. The reduced interface affinity is specific to inhibitor binding because binding the activator ADP at the same allosteric site causes no reduction in subunit affinity. With inhibitor bound, the weakened subunit affinity has allowed the kinetics of dimer association to be elucidated." @default.
• W2079744699 created "2016-06-24" @default.
• W2079744699 creator A5008010294 @default.
• W2079744699 creator A5036608457 @default.
• W2079744699 date "2006-09-01" @default.
• W2079744699 modified "2023-10-16" @default.
• W2079744699 title "Effects of Protein-Ligand Associations on the Subunit Interactions of Phosphofructokinase from <i>B. stearothermophilus</i>" @default.
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• W2079744699 doi "https://doi.org/10.1021/bi0608921" @default.
• W2079744699 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2516970" @default.
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• W2079744699 hasPublicationYear "2006" @default.
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