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- W2079764102 abstract "Proteins of the Enabled/vasodilator-stimulated phosphoprotein (Ena/VASP) family link signal transduction pathways to actin cytoskeleton dynamics. VASP is substrate of cAMP-dependent, cGMP-dependent and AMP-activated protein kinases that primarily phosphorylate the sites S157, S239 and T278, respectively. Here, we systematically analyzed functions of VASP phosphorylation patterns for actin assembly and subcellular targeting in vivo and compared the phosphorylation effects of Ena/VASP family members. Methods used were the reconstitution of VASP-null cells with `locked' phosphomimetic VASP mutants, actin polymerization of VASP mutants in vitro and in living cells, site-specific kinase-mediated VASP phosphorylation, and analysis of the endogenous protein with phosphorylation-status-specific antibodies. Phosphorylation at S157 influenced VASP localization, but had a minor impact on F-actin assembly. Phosphorylation of the S157-equivalent site in the Ena/VASP family members Mena and EVL had no effect on the ratio of cellular F-actin to G-actin. By contrast, VASP phosphorylation at S239 (and the equivalent site in Mena) or T278 impaired VASP-driven actin filament formation. The data show that VASP functions are precisely regulated by differential phosphorylation and provide new insights into cytoskeletal control by serine/threonine kinase-dependent signaling pathways." @default.
- W2079764102 created "2016-06-24" @default.
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- W2079764102 date "2009-11-01" @default.
- W2079764102 modified "2023-10-18" @default.
- W2079764102 title "Differential VASP phosphorylation controls remodeling of the actin cytoskeleton" @default.
- W2079764102 cites W1526841021 @default.
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- W2079764102 cites W1576016596 @default.
- W2079764102 cites W1817574342 @default.
- W2079764102 cites W1969451658 @default.
- W2079764102 cites W1971766102 @default.
- W2079764102 cites W1974326245 @default.
- W2079764102 cites W1974766153 @default.
- W2079764102 cites W1988454624 @default.
- W2079764102 cites W1989992071 @default.
- W2079764102 cites W1995757732 @default.
- W2079764102 cites W1998499089 @default.
- W2079764102 cites W1999663047 @default.
- W2079764102 cites W2002577396 @default.
- W2079764102 cites W2004136067 @default.
- W2079764102 cites W2019787042 @default.
- W2079764102 cites W2020179019 @default.
- W2079764102 cites W2023687572 @default.
- W2079764102 cites W2025036173 @default.
- W2079764102 cites W2033174055 @default.
- W2079764102 cites W2047757962 @default.
- W2079764102 cites W2052764119 @default.
- W2079764102 cites W2053431375 @default.
- W2079764102 cites W2053994471 @default.
- W2079764102 cites W2058416613 @default.
- W2079764102 cites W2063466496 @default.
- W2079764102 cites W2066175662 @default.
- W2079764102 cites W2068972095 @default.
- W2079764102 cites W2069199145 @default.
- W2079764102 cites W2070860745 @default.
- W2079764102 cites W2072242577 @default.
- W2079764102 cites W2073891485 @default.
- W2079764102 cites W2077101231 @default.
- W2079764102 cites W2077489546 @default.
- W2079764102 cites W2083838481 @default.
- W2079764102 cites W2086884747 @default.
- W2079764102 cites W2087677532 @default.
- W2079764102 cites W2090217559 @default.
- W2079764102 cites W2090332465 @default.
- W2079764102 cites W2101873410 @default.
- W2079764102 cites W2119836020 @default.
- W2079764102 cites W2123944878 @default.
- W2079764102 cites W2126120005 @default.
- W2079764102 cites W2128736294 @default.
- W2079764102 cites W2129338565 @default.
- W2079764102 cites W2131200617 @default.
- W2079764102 cites W2133732550 @default.
- W2079764102 cites W2134241152 @default.
- W2079764102 cites W2134535786 @default.
- W2079764102 cites W2137284738 @default.
- W2079764102 cites W2139864298 @default.
- W2079764102 cites W2139969808 @default.
- W2079764102 cites W2148123685 @default.
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- W2079764102 cites W2153423809 @default.
- W2079764102 cites W2158044026 @default.
- W2079764102 cites W2158452258 @default.
- W2079764102 cites W2160307156 @default.
- W2079764102 cites W2160459388 @default.
- W2079764102 cites W2168691181 @default.
- W2079764102 cites W2170602182 @default.
- W2079764102 cites W324436745 @default.
- W2079764102 cites W4213323281 @default.
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- W2079764102 doi "https://doi.org/10.1242/jcs.044537" @default.
- W2079764102 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2773194" @default.
- W2079764102 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19825941" @default.
- W2079764102 hasPublicationYear "2009" @default.
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