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- W2079771239 abstract "The effects of purified FSH preparations from three different species (ovine, human, rat) on the steroidogenic pattern in isolated pre-ovulatory follicles of PMSG-treated immature rats were examined and compared to the effects of LH (ovine, human). Steroids were analyzed by RIA. During 6 h incubation, all three FSH preparations in a concentration range from 10-100 ng/ml caused a significant increase in progesterone (P), testosterone (T), and oestradiol (E2) accumulation. With LH a significant increase in steroid accumulation was seen in a concentration of 1 ng/ml. The time-course of stimulation by hFSH and hLH in a concentration of 10 ng/ml showed no difference in steroidogenic response between the two hormones, with a significant stimulation of steroids within 2 h. During prolonged incubation (6-8 h), accumulation of T was measured in the presence or absence of unlabelled 17 alpha-hydroxyprogesterone. Follicles exposed to LH did not increase their T formation, while follicles incubated in plain medium or in the presence of oFSH increased their T formation. In a concentration of 100 ng/ml hFSH showed a tendency to reduce maximal T production. These results suggest that FSH alone in physiological doses may stimulate T and E2 production in the preovulatory follicle." @default.
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- W2079771239 date "1985-04-01" @default.
- W2079771239 modified "2023-09-26" @default.
- W2079771239 title "Comparison between the effects of FSH and LH on the steroidogenic pattern in isolated pre-ovulatory rat follicles" @default.
- W2079771239 doi "https://doi.org/10.1530/acta.0.1080557" @default.
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