FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079796553> ?p ?o ?g. }

• W2079796553 endingPage "19598" @default.
• W2079796553 startingPage "19593" @default.
• W2079796553 abstract "Dihydrouridine (D) is a highly conserved modified base found in tRNAs from all domains of life. Dihydrouridine synthase (Dus) catalyzes the D formation of tRNA through reduction of uracil base with flavin mononucleotide (FMN) as a cofactor. Here, we report the crystal structures of Thermus thermophilus Dus ( Tth Dus), which is responsible for D formation at positions 20 and 20a, in complex with tRNA and with a short fragment of tRNA (D-loop). Dus interacts extensively with the D-arm and recognizes the elbow region composed of the kissing loop interaction between T- and D-loops in tRNA, pulling U20 into the catalytic center for reduction. Although distortion of the D-loop structure was observed upon binding of Dus to tRNA, the canonical D-loop/T-loop interaction was maintained. These results were consistent with the observation that Dus preferentially recognizes modified rather than unmodified tRNAs, indicating that Dus introduces D20 by monitoring the complete L-shaped structure of tRNAs. In the active site, U20 is stacked on the isoalloxazine ring of FMN, and C5 of the U20 uracil ring is covalently cross linked to the thiol group of Cys93, implying a catalytic mechanism of D20 formation. In addition, the involvement of a cofactor molecule in uracil ring recognition was proposed. Based on a series of mutation analyses, we propose a molecular basis of tRNA recognition and D formation catalyzed by Dus." @default.
• W2079796553 created "2016-06-24" @default.
• W2079796553 creator A5001653266 @default.
• W2079796553 creator A5010384396 @default.
• W2079796553 creator A5020556619 @default.
• W2079796553 creator A5022233637 @default.
• W2079796553 creator A5022472105 @default.
• W2079796553 creator A5046259804 @default.
• W2079796553 creator A5077307889 @default.
• W2079796553 creator A5083249193 @default.
• W2079796553 creator A5089024312 @default.
• W2079796553 date "2011-11-28" @default.
• W2079796553 modified "2023-10-01" @default.
• W2079796553 title "Molecular basis of dihydrouridine formation on tRNA" @default.
• W2079796553 cites W1969929356 @default.
• W2079796553 cites W1975372074 @default.
• W2079796553 cites W1996630340 @default.
• W2079796553 cites W2026004038 @default.
• W2079796553 cites W2032407233 @default.
• W2079796553 cites W2044064939 @default.
• W2079796553 cites W2058877183 @default.
• W2079796553 cites W2058913943 @default.
• W2079796553 cites W2064367668 @default.
• W2079796553 cites W2074858412 @default.
• W2079796553 cites W2087158203 @default.
• W2079796553 cites W2089204784 @default.
• W2079796553 cites W2090837206 @default.
• W2079796553 cites W2094729320 @default.
• W2079796553 cites W2099546395 @default.
• W2079796553 cites W2100353926 @default.
• W2079796553 cites W2109446129 @default.
• W2079796553 cites W2114478647 @default.
• W2079796553 cites W2133168873 @default.
• W2079796553 cites W2133666260 @default.
• W2079796553 cites W2155376412 @default.
• W2079796553 cites W2157548679 @default.
• W2079796553 cites W4234887284 @default.
• W2079796553 doi "https://doi.org/10.1073/pnas.1112352108" @default.
• W2079796553 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3241823" @default.
• W2079796553 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22123979" @default.
• W2079796553 hasPublicationYear "2011" @default.
• W2079796553 type Work @default.
• W2079796553 sameAs 2079796553 @default.
• W2079796553 citedByCount "53" @default.
• W2079796553 countsByYear W20797965532012 @default.
• W2079796553 countsByYear W20797965532013 @default.
• W2079796553 countsByYear W20797965532014 @default.
• W2079796553 countsByYear W20797965532015 @default.
• W2079796553 countsByYear W20797965532016 @default.
• W2079796553 countsByYear W20797965532017 @default.
• W2079796553 countsByYear W20797965532018 @default.
• W2079796553 countsByYear W20797965532019 @default.
• W2079796553 countsByYear W20797965532020 @default.
• W2079796553 countsByYear W20797965532021 @default.
• W2079796553 countsByYear W20797965532022 @default.
• W2079796553 countsByYear W20797965532023 @default.
• W2079796553 crossrefType "journal-article" @default.
• W2079796553 hasAuthorship W2079796553A5001653266 @default.
• W2079796553 hasAuthorship W2079796553A5010384396 @default.
• W2079796553 hasAuthorship W2079796553A5020556619 @default.
• W2079796553 hasAuthorship W2079796553A5022233637 @default.
• W2079796553 hasAuthorship W2079796553A5022472105 @default.
• W2079796553 hasAuthorship W2079796553A5046259804 @default.
• W2079796553 hasAuthorship W2079796553A5077307889 @default.
• W2079796553 hasAuthorship W2079796553A5083249193 @default.
• W2079796553 hasAuthorship W2079796553A5089024312 @default.
• W2079796553 hasBestOaLocation W20797965531 @default.
• W2079796553 hasConcept C104317684 @default.
• W2079796553 hasConcept C153957851 @default.
• W2079796553 hasConcept C178790620 @default.
• W2079796553 hasConcept C181199279 @default.
• W2079796553 hasConcept C185592680 @default.
• W2079796553 hasConcept C197957613 @default.
• W2079796553 hasConcept C2777427158 @default.
• W2079796553 hasConcept C2779554091 @default.
• W2079796553 hasConcept C2780378348 @default.
• W2079796553 hasConcept C2780883830 @default.
• W2079796553 hasConcept C547475151 @default.
• W2079796553 hasConcept C552990157 @default.
• W2079796553 hasConcept C55493867 @default.
• W2079796553 hasConcept C67705224 @default.
• W2079796553 hasConcept C71240020 @default.
• W2079796553 hasConcept C86803240 @default.
• W2079796553 hasConceptScore W2079796553C104317684 @default.
• W2079796553 hasConceptScore W2079796553C153957851 @default.
• W2079796553 hasConceptScore W2079796553C178790620 @default.
• W2079796553 hasConceptScore W2079796553C181199279 @default.
• W2079796553 hasConceptScore W2079796553C185592680 @default.
• W2079796553 hasConceptScore W2079796553C197957613 @default.
• W2079796553 hasConceptScore W2079796553C2777427158 @default.
• W2079796553 hasConceptScore W2079796553C2779554091 @default.
• W2079796553 hasConceptScore W2079796553C2780378348 @default.
• W2079796553 hasConceptScore W2079796553C2780883830 @default.
• W2079796553 hasConceptScore W2079796553C547475151 @default.
• W2079796553 hasConceptScore W2079796553C552990157 @default.
• W2079796553 hasConceptScore W2079796553C55493867 @default.
• W2079796553 hasConceptScore W2079796553C67705224 @default.
• W2079796553 hasConceptScore W2079796553C71240020 @default.

• next