FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079799897> ?p ?o ?g. }

• W2079799897 endingPage "485" @default.
• W2079799897 startingPage "480" @default.
• W2079799897 abstract "Summary Background Basophils are increasingly utilized as indicators of allergic inflammation and as primary allergic effector cells to study signalling pathways. However, until the present, their enrichment has been time consuming, costly and limited to relatively few specialized laboratories. Objective We have therefore devised a reproducible and rapid method for the purification of human basophils from small quantities of peripheral blood within 1.5 h, which does not require the use of specialized equipment such as elutriators. Methods Human basophils were obtained from healthy volunteers undergoing venipuncture. Heparinized or K3‐ethylenediaminetetraacetic acid blood samples were first subjected to centrifugation in Hetasep, directly followed by negative selection using immunomagnetic beads. Basophil morphology and purity were assessed by May–Grünwald staining of cytospins. IgE‐mediated histamine release was analysed spectrofluorometrically and IL‐4 and IL‐13 production by quantitative RT‐PCR. CD203c and CD63 surface expression was measured using flow cytometry before and after activation with anti‐IgE. Results Using this protocol, basophils were enriched close to homogeneity in most cases with a mean purity of 99.34±0.88% (range 97–100%, n =18) and a mean recovery of 75.6 (range 39–100%, n =8). Basophil viability following purification was 99.6±0.89% using Trypan blue exclusion. The purification procedure gave rise to basophils with normal functional responses to anti‐IgE regarding histamine release as well as IL‐4 and IL‐13 mRNA expression. Moreover, constitutive cell‐surface CD203c/CD63 expressions were not elevated before anti‐IgE stimulation. Conclusion The rapidity, simplicity and reproducibility of this method will facilitate the employment of basophils in high‐output ex vivo studies." @default.
• W2079799897 created "2016-06-24" @default.
• W2079799897 creator A5011732165 @default.
• W2079799897 creator A5078531159 @default.
• W2079799897 creator A5083133805 @default.
• W2079799897 date "2008-01-14" @default.
• W2079799897 modified "2023-10-15" @default.
• W2079799897 title "A rapid two-step procedure for the purification of human peripheral blood basophils to near homogeneity" @default.
• W2079799897 cites W1595101033 @default.
• W2079799897 cites W1964136853 @default.
• W2079799897 cites W1979608756 @default.
• W2079799897 cites W1983852365 @default.
• W2079799897 cites W1992295258 @default.
• W2079799897 cites W2005989655 @default.
• W2079799897 cites W2019450931 @default.
• W2079799897 cites W2022017784 @default.
• W2079799897 cites W2027040241 @default.
• W2079799897 cites W2036567725 @default.
• W2079799897 cites W2088337800 @default.
• W2079799897 cites W2108244474 @default.
• W2079799897 cites W2124824811 @default.
• W2079799897 cites W2125918565 @default.
• W2079799897 cites W2160472622 @default.
• W2079799897 cites W2731228282 @default.
• W2079799897 doi "https://doi.org/10.1111/j.1365-2222.2007.02919.x" @default.
• W2079799897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18201250" @default.
• W2079799897 hasPublicationYear "2008" @default.
• W2079799897 type Work @default.
• W2079799897 sameAs 2079799897 @default.
• W2079799897 citedByCount "33" @default.
• W2079799897 countsByYear W20797998972012 @default.
• W2079799897 countsByYear W20797998972013 @default.
• W2079799897 countsByYear W20797998972014 @default.
• W2079799897 countsByYear W20797998972015 @default.
• W2079799897 countsByYear W20797998972016 @default.
• W2079799897 countsByYear W20797998972017 @default.
• W2079799897 countsByYear W20797998972018 @default.
• W2079799897 countsByYear W20797998972019 @default.
• W2079799897 countsByYear W20797998972020 @default.
• W2079799897 countsByYear W20797998972022 @default.
• W2079799897 countsByYear W20797998972023 @default.
• W2079799897 crossrefType "journal-article" @default.
• W2079799897 hasAuthorship W2079799897A5011732165 @default.
• W2079799897 hasAuthorship W2079799897A5078531159 @default.
• W2079799897 hasAuthorship W2079799897A5083133805 @default.
• W2079799897 hasConcept C104317684 @default.
• W2079799897 hasConcept C1122143 @default.
• W2079799897 hasConcept C126322002 @default.
• W2079799897 hasConcept C141105273 @default.
• W2079799897 hasConcept C145059251 @default.
• W2079799897 hasConcept C153911025 @default.
• W2079799897 hasConcept C159654299 @default.
• W2079799897 hasConcept C1621761 @default.
• W2079799897 hasConcept C16685009 @default.
• W2079799897 hasConcept C185592680 @default.
• W2079799897 hasConcept C203014093 @default.
• W2079799897 hasConcept C20518536 @default.
• W2079799897 hasConcept C2776385711 @default.
• W2079799897 hasConcept C2777456892 @default.
• W2079799897 hasConcept C2779176990 @default.
• W2079799897 hasConcept C42219234 @default.
• W2079799897 hasConcept C43617362 @default.
• W2079799897 hasConcept C553184892 @default.
• W2079799897 hasConcept C55493867 @default.
• W2079799897 hasConcept C71924100 @default.
• W2079799897 hasConcept C74414138 @default.
• W2079799897 hasConcept C7623868 @default.
• W2079799897 hasConcept C86803240 @default.
• W2079799897 hasConceptScore W2079799897C104317684 @default.
• W2079799897 hasConceptScore W2079799897C1122143 @default.
• W2079799897 hasConceptScore W2079799897C126322002 @default.
• W2079799897 hasConceptScore W2079799897C141105273 @default.
• W2079799897 hasConceptScore W2079799897C145059251 @default.
• W2079799897 hasConceptScore W2079799897C153911025 @default.
• W2079799897 hasConceptScore W2079799897C159654299 @default.
• W2079799897 hasConceptScore W2079799897C1621761 @default.
• W2079799897 hasConceptScore W2079799897C16685009 @default.
• W2079799897 hasConceptScore W2079799897C185592680 @default.
• W2079799897 hasConceptScore W2079799897C203014093 @default.
• W2079799897 hasConceptScore W2079799897C20518536 @default.
• W2079799897 hasConceptScore W2079799897C2776385711 @default.
• W2079799897 hasConceptScore W2079799897C2777456892 @default.
• W2079799897 hasConceptScore W2079799897C2779176990 @default.
• W2079799897 hasConceptScore W2079799897C42219234 @default.
• W2079799897 hasConceptScore W2079799897C43617362 @default.
• W2079799897 hasConceptScore W2079799897C553184892 @default.
• W2079799897 hasConceptScore W2079799897C55493867 @default.
• W2079799897 hasConceptScore W2079799897C71924100 @default.
• W2079799897 hasConceptScore W2079799897C74414138 @default.
• W2079799897 hasConceptScore W2079799897C7623868 @default.
• W2079799897 hasConceptScore W2079799897C86803240 @default.
• W2079799897 hasIssue "3" @default.
• W2079799897 hasLocation W20797998971 @default.
• W2079799897 hasLocation W20797998972 @default.
• W2079799897 hasOpenAccess W2079799897 @default.
• W2079799897 hasPrimaryLocation W20797998971 @default.
• W2079799897 hasRelatedWork W1974675198 @default.
• W2079799897 hasRelatedWork W2012769899 @default.

• next