FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079821886> ?p ?o ?g. }

• W2079821886 endingPage "1139" @default.
• W2079821886 startingPage "1130" @default.
• W2079821886 abstract "Pertussis toxin (PT), an AB5 exotoxin and important virulence factor of Bordetella pertussis, is hypothesized to traffic along a retrograde transport pathway in mammalian cells. This pathway includes endosomal uptake, transport to the Golgi complex and endoplasmic reticulum (ER), dissociation of the holotoxin in the ER and translocation of the A subunit (S1) to the cytosol, where it ADP-ribosylates its G protein targets. In this study, PT was visualized in the Golgi complex by immunofluorescence microscopy, but transport beyond the Golgi could not be detected by this method. To gain evidence for the retrograde pathway, peptide tags with target sites for tyrosine sulfation (a trans-Golgi network-specific activity) and N-glycosylation (an ER-specific activity) were added to either S1 or a B subunit (S4) of PT. Modified PT retained in vitro enzymatic and cellular activity as assessed by ADP-ribosylation assays. Peptide-tagged PT subunits were found to be modified by tyrosine sulfation, and, at later time points, by N-glycosylation. Appearance of sulfated PT subunits was inhibited by pretreatment of cells with brefeldin A. In some cell types, much of the S4 glycosylation, but not that of S1, was resistant to endoglycosidase H, suggesting that, subsequent to core N-glycosylation in the ER, S4 was transported anterograde to the Golgi, where further glycosylation occurred. When cells were pretreated with methyl-beta-cyclodextrin, sulfation of PT subunits and PT cytotoxicity were reduced, suggesting that PT transport is dependent on cellular cholesterol content. These data support a retrograde pathway for PT intracellular transport." @default.
• W2079821886 created "2016-06-24" @default.
• W2079821886 creator A5075325149 @default.
• W2079821886 creator A5077507710 @default.
• W2079821886 date "2008-05-01" @default.
• W2079821886 modified "2023-10-12" @default.
• W2079821886 title "Retrograde transport of pertussis toxin in the mammalian cell" @default.
• W2079821886 cites W1029261710 @default.
• W2079821886 cites W1565287219 @default.
• W2079821886 cites W1593869902 @default.
• W2079821886 cites W1602962366 @default.
• W2079821886 cites W1889094273 @default.
• W2079821886 cites W1912655461 @default.
• W2079821886 cites W1950084933 @default.
• W2079821886 cites W1962485516 @default.
• W2079821886 cites W1968774879 @default.
• W2079821886 cites W1977498240 @default.
• W2079821886 cites W1980775568 @default.
• W2079821886 cites W1982675242 @default.
• W2079821886 cites W1987804131 @default.
• W2079821886 cites W1994437994 @default.
• W2079821886 cites W2004625433 @default.
• W2079821886 cites W2016871586 @default.
• W2079821886 cites W2023531893 @default.
• W2079821886 cites W2031119708 @default.
• W2079821886 cites W2043178667 @default.
• W2079821886 cites W2045249005 @default.
• W2079821886 cites W2047034219 @default.
• W2079821886 cites W2047246412 @default.
• W2079821886 cites W2050120655 @default.
• W2079821886 cites W2056239892 @default.
• W2079821886 cites W2058997748 @default.
• W2079821886 cites W2060913542 @default.
• W2079821886 cites W2065105668 @default.
• W2079821886 cites W2071021491 @default.
• W2079821886 cites W2071717900 @default.
• W2079821886 cites W2073038487 @default.
• W2079821886 cites W2090685385 @default.
• W2079821886 cites W2096623610 @default.
• W2079821886 cites W2102037519 @default.
• W2079821886 cites W2104596749 @default.
• W2079821886 cites W2122796455 @default.
• W2079821886 cites W2130388575 @default.
• W2079821886 cites W2135511693 @default.
• W2079821886 cites W2136344073 @default.
• W2079821886 cites W2140115692 @default.
• W2079821886 cites W2150390278 @default.
• W2079821886 cites W2152649267 @default.
• W2079821886 cites W2155588974 @default.
• W2079821886 cites W2156145475 @default.
• W2079821886 cites W2160942713 @default.
• W2079821886 cites W2260855040 @default.
• W2079821886 cites W962059363 @default.
• W2079821886 doi "https://doi.org/10.1111/j.1462-5822.2007.01115.x" @default.
• W2079821886 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18201245" @default.
• W2079821886 hasPublicationYear "2008" @default.
• W2079821886 type Work @default.
• W2079821886 sameAs 2079821886 @default.
• W2079821886 citedByCount "65" @default.
• W2079821886 countsByYear W20798218862012 @default.
• W2079821886 countsByYear W20798218862013 @default.
• W2079821886 countsByYear W20798218862014 @default.
• W2079821886 countsByYear W20798218862015 @default.
• W2079821886 countsByYear W20798218862016 @default.
• W2079821886 countsByYear W20798218862017 @default.
• W2079821886 countsByYear W20798218862018 @default.
• W2079821886 countsByYear W20798218862019 @default.
• W2079821886 countsByYear W20798218862020 @default.
• W2079821886 countsByYear W20798218862021 @default.
• W2079821886 countsByYear W20798218862022 @default.
• W2079821886 countsByYear W20798218862023 @default.
• W2079821886 crossrefType "journal-article" @default.
• W2079821886 hasAuthorship W2079821886A5075325149 @default.
• W2079821886 hasAuthorship W2079821886A5077507710 @default.
• W2079821886 hasBestOaLocation W20798218861 @default.
• W2079821886 hasConcept C102747710 @default.
• W2079821886 hasConcept C119062480 @default.
• W2079821886 hasConcept C158617107 @default.
• W2079821886 hasConcept C2777313579 @default.
• W2079821886 hasConcept C2779571893 @default.
• W2079821886 hasConcept C2780654058 @default.
• W2079821886 hasConcept C2781373781 @default.
• W2079821886 hasConcept C49773422 @default.
• W2079821886 hasConcept C55493867 @default.
• W2079821886 hasConcept C62478195 @default.
• W2079821886 hasConcept C79879829 @default.
• W2079821886 hasConcept C80631254 @default.
• W2079821886 hasConcept C86803240 @default.
• W2079821886 hasConcept C95444343 @default.
• W2079821886 hasConceptScore W2079821886C102747710 @default.
• W2079821886 hasConceptScore W2079821886C119062480 @default.
• W2079821886 hasConceptScore W2079821886C158617107 @default.
• W2079821886 hasConceptScore W2079821886C2777313579 @default.
• W2079821886 hasConceptScore W2079821886C2779571893 @default.
• W2079821886 hasConceptScore W2079821886C2780654058 @default.
• W2079821886 hasConceptScore W2079821886C2781373781 @default.
• W2079821886 hasConceptScore W2079821886C49773422 @default.
• W2079821886 hasConceptScore W2079821886C55493867 @default.

• next