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- W2079832964 abstract "The adjuvant effect of liposomes formulated with three phospholipids including phosphatidylcholine-liposomes (PC-Lip), phosphatidylserine-liposomes (PS-Lip), and stearylamine-liposomes (SA-Lip) was compared with virus alone using inactivated Newcastle disease virus (NDV) as a model antigen. The difference in adjuvanticity was evaluated using the haemagglutination-inhibition (HI) test, enzyme-linked immunosorbent assay, and a challenge study following intranasal inoculation of specific pathogen-free chickens. After two inoculations, a liposomal vaccine consisting of NDV in PC-Lip resulted in a significant increase in HI titre, up to 32-fold higher than a vaccine containing virus alone and 320-fold higher than a vaccine containing NDV in SA-Lip. PC-Lip also elicited a significant mucosal secretary immunoglobulin A response (P < 0.05) in tracheal lavages and a serum IgG response (P < 0.05). In response to viral challenge, all control animals died, whereas 90% of animals which received PC-Lip survived. The results suggest that PC-Lip may be suitable as an adjuvant for mucosal vaccination against NDV in chickens." @default.
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- W2079832964 date "2010-08-01" @default.
- W2079832964 modified "2023-10-18" @default.
- W2079832964 title "The influence of liposomal adjuvant on intranasal vaccination of chickens against Newcastle disease" @default.
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- W2079832964 doi "https://doi.org/10.1016/j.tvjl.2009.05.019" @default.
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