Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079837524> ?p ?o ?g. }
- W2079837524 endingPage "439" @default.
- W2079837524 startingPage "430" @default.
- W2079837524 abstract "Objective Hypoxic culture potentiates mesenchymal stem cells ( MSC s) to survive and secrete various growth factors. Genetically modified stem cells overexpressing bone morphogenic protein‐2 ( BMP ‐2) demonstrate strong osteogenic ability. Hence, we investigated the coeffect of hypoxic culture conditions and BMP ‐2 overexpression on the osteogenic ability of rabbit adipose‐derived stem cells (r ASC s) in vitro . Materials and Methods Rabbit adipose‐derived stem cells with or without adenoviral‐ BMP ‐2 transduction were cultured in hypoxic (1%) and normoxic (21%) conditions. Cell viability, attachment, and proliferation were compared. Real‐time PCR amplification of osteogenic and angiogenic genes including alkaline phosphatase ( ALP ), osteocalcin ( OCN ), HIF ‐1α, and vascular endothelial growth factor ( VEGF ) was performed. Moreover, ALP activity, immunofluorescent staining of OCN , and mineralization assay by alizarin red S quantification and von K ossa staining were conducted. Results Cells under hypoxic conditions attached better within 12 h and proliferated faster. While BMP ‐2 overexpression and hypoxic condition separately elevated the transcription of key osteogenic and angiogenic genes, a cooperative effect was observed to enhance the upregulation of osteogenic as well as angiogenic genes. Identical changes were observed in ALP activity, immunofluorescent staining of OCN , and mineralization assay. Conclusions Hypoxic culture can enhance the osteogenic ability of BMP ‐2 gene‐modified r ASC s, which provides a strategy to improve the osteogenesis of r ASC s for in vivo bone regeneration." @default.
- W2079837524 created "2016-06-24" @default.
- W2079837524 creator A5005782389 @default.
- W2079837524 creator A5006533811 @default.
- W2079837524 creator A5016680388 @default.
- W2079837524 creator A5042518913 @default.
- W2079837524 creator A5045061140 @default.
- W2079837524 creator A5048077073 @default.
- W2079837524 creator A5054380620 @default.
- W2079837524 creator A5069075917 @default.
- W2079837524 creator A5078402710 @default.
- W2079837524 creator A5080423598 @default.
- W2079837524 date "2013-07-19" @default.
- W2079837524 modified "2023-10-17" @default.
- W2079837524 title "Hypoxia induces osteogenesis in rabbit adipose‐derived stem cells overexpressing bone morphogenic protein‐2" @default.
- W2079837524 cites W1578283200 @default.
- W2079837524 cites W1963922762 @default.
- W2079837524 cites W1964439844 @default.
- W2079837524 cites W1964802392 @default.
- W2079837524 cites W1976620205 @default.
- W2079837524 cites W1979793536 @default.
- W2079837524 cites W1982861439 @default.
- W2079837524 cites W1985647009 @default.
- W2079837524 cites W1985721879 @default.
- W2079837524 cites W1993925802 @default.
- W2079837524 cites W1993943657 @default.
- W2079837524 cites W2000038050 @default.
- W2079837524 cites W2003649275 @default.
- W2079837524 cites W2004072752 @default.
- W2079837524 cites W2005964931 @default.
- W2079837524 cites W2015136076 @default.
- W2079837524 cites W2025736528 @default.
- W2079837524 cites W2025838486 @default.
- W2079837524 cites W2028153000 @default.
- W2079837524 cites W2031774583 @default.
- W2079837524 cites W2038607522 @default.
- W2079837524 cites W2043814008 @default.
- W2079837524 cites W2044322920 @default.
- W2079837524 cites W2044760997 @default.
- W2079837524 cites W2051138615 @default.
- W2079837524 cites W2056007437 @default.
- W2079837524 cites W2060080286 @default.
- W2079837524 cites W2063011340 @default.
- W2079837524 cites W2066954473 @default.
- W2079837524 cites W2067874044 @default.
- W2079837524 cites W2068831893 @default.
- W2079837524 cites W2074121232 @default.
- W2079837524 cites W2078723690 @default.
- W2079837524 cites W2081868893 @default.
- W2079837524 cites W2083456009 @default.
- W2079837524 cites W2084980999 @default.
- W2079837524 cites W2089530989 @default.
- W2079837524 cites W2096026776 @default.
- W2079837524 cites W2107576625 @default.
- W2079837524 cites W2110234623 @default.
- W2079837524 cites W2113051470 @default.
- W2079837524 cites W2121927581 @default.
- W2079837524 cites W2129336783 @default.
- W2079837524 cites W2138007542 @default.
- W2079837524 cites W2139237341 @default.
- W2079837524 cites W2141881387 @default.
- W2079837524 cites W2150350398 @default.
- W2079837524 cites W2156820957 @default.
- W2079837524 cites W2166764740 @default.
- W2079837524 cites W2170074752 @default.
- W2079837524 cites W2171965603 @default.
- W2079837524 doi "https://doi.org/10.1111/odi.12148" @default.
- W2079837524 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23865899" @default.
- W2079837524 hasPublicationYear "2013" @default.
- W2079837524 type Work @default.
- W2079837524 sameAs 2079837524 @default.
- W2079837524 citedByCount "14" @default.
- W2079837524 countsByYear W20798375242015 @default.
- W2079837524 countsByYear W20798375242016 @default.
- W2079837524 countsByYear W20798375242018 @default.
- W2079837524 countsByYear W20798375242019 @default.
- W2079837524 countsByYear W20798375242020 @default.
- W2079837524 countsByYear W20798375242021 @default.
- W2079837524 countsByYear W20798375242022 @default.
- W2079837524 countsByYear W20798375242023 @default.
- W2079837524 crossrefType "journal-article" @default.
- W2079837524 hasAuthorship W2079837524A5005782389 @default.
- W2079837524 hasAuthorship W2079837524A5006533811 @default.
- W2079837524 hasAuthorship W2079837524A5016680388 @default.
- W2079837524 hasAuthorship W2079837524A5042518913 @default.
- W2079837524 hasAuthorship W2079837524A5045061140 @default.
- W2079837524 hasAuthorship W2079837524A5048077073 @default.
- W2079837524 hasAuthorship W2079837524A5054380620 @default.
- W2079837524 hasAuthorship W2079837524A5069075917 @default.
- W2079837524 hasAuthorship W2079837524A5078402710 @default.
- W2079837524 hasAuthorship W2079837524A5080423598 @default.
- W2079837524 hasConcept C131075544 @default.
- W2079837524 hasConcept C153911025 @default.
- W2079837524 hasConcept C160160445 @default.
- W2079837524 hasConcept C171089720 @default.
- W2079837524 hasConcept C181199279 @default.
- W2079837524 hasConcept C185592680 @default.
- W2079837524 hasConcept C198826908 @default.