Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079838383> ?p ?o ?g. }
- W2079838383 endingPage "50" @default.
- W2079838383 startingPage "45" @default.
- W2079838383 abstract "Tight junctions play a pivotal role in maintaining the integrity of the intestinal barrier. Their alteration is involved in the pathogenesis of celiac disease. Our aim was to investigate the gliadin effect on the tight junction proteins in an in vitro three-dimensional cell culture model through imaging analyses. Lovo multicellular spheroids were treated with enzymatically digested (PT) gliadin 500 μg/mL and its effect on actin, occludin and zonula occludens-1, was evaluated by means of confocal laser microscopy, transmission electron microscopy and image capture analysis. Compared to untreated spheroids, PT-gliadin-treated ones showed enlargement of the paracellular spaces (9.0±6.9 vs. 6.2±1.7 nm, p<0.05) at transmission electron microscopy and tight junction protein alterations at confocal microscopy and image analyses. In untreated cell cultures thickness of the fluorescence contour of actin, zonula occludens-1 and occludin appeared significantly larger and more intense than in the treated ones. In occludin planimetric analysis the lengths of the integral uninterrupted cellular contour appeared longer in untreated than in PT-gliadin treated spheroids (71.8±42.8 vs. 23.4±25.9 μm, p<0.01). Our data demonstrated that tight junction proteins are directly damaged by gliadin as shown by means of quantitative imaging analysis." @default.
- W2079838383 created "2016-06-24" @default.
- W2079838383 creator A5003256299 @default.
- W2079838383 creator A5022487056 @default.
- W2079838383 creator A5052333697 @default.
- W2079838383 creator A5053796845 @default.
- W2079838383 creator A5072368737 @default.
- W2079838383 creator A5077151164 @default.
- W2079838383 creator A5077963483 @default.
- W2079838383 creator A5085900234 @default.
- W2079838383 date "2011-02-01" @default.
- W2079838383 modified "2023-09-23" @default.
- W2079838383 title "Imaging analysis of the gliadin direct effect on tight junctions in an in vitro three-dimensional Lovo cell line culture system" @default.
- W2079838383 cites W1584256905 @default.
- W2079838383 cites W1971554225 @default.
- W2079838383 cites W1992369970 @default.
- W2079838383 cites W1994349104 @default.
- W2079838383 cites W1994726850 @default.
- W2079838383 cites W2001487770 @default.
- W2079838383 cites W2006194008 @default.
- W2079838383 cites W2014662185 @default.
- W2079838383 cites W2028622825 @default.
- W2079838383 cites W2035102361 @default.
- W2079838383 cites W2040077904 @default.
- W2079838383 cites W2041549174 @default.
- W2079838383 cites W2041733088 @default.
- W2079838383 cites W2043886619 @default.
- W2079838383 cites W2058036417 @default.
- W2079838383 cites W2069466944 @default.
- W2079838383 cites W2074150842 @default.
- W2079838383 cites W2111994809 @default.
- W2079838383 cites W2122846253 @default.
- W2079838383 cites W2129361296 @default.
- W2079838383 cites W2144178838 @default.
- W2079838383 cites W2150267721 @default.
- W2079838383 cites W2150771988 @default.
- W2079838383 cites W2155579605 @default.
- W2079838383 cites W2161266638 @default.
- W2079838383 cites W2165199863 @default.
- W2079838383 cites W2167464199 @default.
- W2079838383 cites W2285654847 @default.
- W2079838383 cites W4236836788 @default.
- W2079838383 cites W4250158686 @default.
- W2079838383 doi "https://doi.org/10.1016/j.tiv.2010.09.005" @default.
- W2079838383 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20850517" @default.
- W2079838383 hasPublicationYear "2011" @default.
- W2079838383 type Work @default.
- W2079838383 sameAs 2079838383 @default.
- W2079838383 citedByCount "13" @default.
- W2079838383 countsByYear W20798383832012 @default.
- W2079838383 countsByYear W20798383832013 @default.
- W2079838383 countsByYear W20798383832015 @default.
- W2079838383 countsByYear W20798383832017 @default.
- W2079838383 countsByYear W20798383832018 @default.
- W2079838383 countsByYear W20798383832019 @default.
- W2079838383 countsByYear W20798383832021 @default.
- W2079838383 countsByYear W20798383832022 @default.
- W2079838383 crossrefType "journal-article" @default.
- W2079838383 hasAuthorship W2079838383A5003256299 @default.
- W2079838383 hasAuthorship W2079838383A5022487056 @default.
- W2079838383 hasAuthorship W2079838383A5052333697 @default.
- W2079838383 hasAuthorship W2079838383A5053796845 @default.
- W2079838383 hasAuthorship W2079838383A5072368737 @default.
- W2079838383 hasAuthorship W2079838383A5077151164 @default.
- W2079838383 hasAuthorship W2079838383A5077963483 @default.
- W2079838383 hasAuthorship W2079838383A5085900234 @default.
- W2079838383 hasConcept C120665830 @default.
- W2079838383 hasConcept C121332964 @default.
- W2079838383 hasConcept C12554922 @default.
- W2079838383 hasConcept C136009344 @default.
- W2079838383 hasConcept C142724271 @default.
- W2079838383 hasConcept C146088050 @default.
- W2079838383 hasConcept C147080431 @default.
- W2079838383 hasConcept C1491633281 @default.
- W2079838383 hasConcept C169274487 @default.
- W2079838383 hasConcept C171250308 @default.
- W2079838383 hasConcept C177779419 @default.
- W2079838383 hasConcept C185592680 @default.
- W2079838383 hasConcept C192562407 @default.
- W2079838383 hasConcept C202751555 @default.
- W2079838383 hasConcept C2775962179 @default.
- W2079838383 hasConcept C2776336767 @default.
- W2079838383 hasConcept C2779178360 @default.
- W2079838383 hasConcept C2780092158 @default.
- W2079838383 hasConcept C29350253 @default.
- W2079838383 hasConcept C51872919 @default.
- W2079838383 hasConcept C54355233 @default.
- W2079838383 hasConcept C55493867 @default.
- W2079838383 hasConcept C71924100 @default.
- W2079838383 hasConcept C81885089 @default.
- W2079838383 hasConcept C86803240 @default.
- W2079838383 hasConcept C91881484 @default.
- W2079838383 hasConcept C93877712 @default.
- W2079838383 hasConcept C95444343 @default.
- W2079838383 hasConceptScore W2079838383C120665830 @default.
- W2079838383 hasConceptScore W2079838383C121332964 @default.
- W2079838383 hasConceptScore W2079838383C12554922 @default.
- W2079838383 hasConceptScore W2079838383C136009344 @default.