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- W2079841246 abstract "The 5' end of the upstream regulatory region (URR) of human papillomavirus type 11 (HPV-11) has enhancer function and binds cellular proteins. We have determined that one particular motif, a CCNGTNAC pair, is both necessary and sufficient for enhancer activity. This enhancement of expression can be competed in vivo by concatenated double-stranded oligonucleotides, indicating that protein-DNA binding is a requisite for enhancer activity. A 41-kDa protein, present in all epithelial cells assayed, binds to this enhancer motif. The 5'URR fragment functions as an enhancer both in primary keratinocytes from a variety of body sites and in fibroblasts. We conclude that tissue specificity is not a feature of this enhancer, and that the 41-kDa binding protein is ubiquitous. These data provide evidence that the 5'URR enhancer activity is dependent on only a few sequences and perhaps only one protein." @default.
- W2079841246 created "2016-06-24" @default.
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- W2079841246 date "1991-03-01" @default.
- W2079841246 modified "2023-10-16" @default.
- W2079841246 title "A key DNA-protein interaction determines the function of the 5′URR enhancer in human papillomavirus type 11" @default.
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- W2079841246 doi "https://doi.org/10.1016/0042-6822(91)90477-s" @default.
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