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- W2079866619 abstract "Protein-protein interactions regulate biological processes and are fundamental for cell functions. Recently, efforts have been made to define interactomes, which are maps of protein-protein interactions that are useful for understanding biological pathways and networks and for investigating how perturbations of these networks lead to diseases. Therefore, interactomes are becoming fundamental for establishing the molecular basis of human diseases and contributing to the discovery of effective therapies. Interactomes are constructed based on experimental data present in the literature and computational predictions of interactions. Several biochemical, genetic and biotechnological techniques have been used in the past to identify protein-protein interactions. The yeast two-hybrid system has beyond doubt represented a revolution in the field, being a versatile tool and allowing the immediate identification of the interacting proteins and isolation of the cDNA coding for the interacting peptide after in vivo screening. Recently, variants of the yeast two-hybrid assay have been developed, including high-throughput systems that promote the rapidly growing field of proteomics. In this review we will focus on the role of this technique in the discovery of Rab interacting proteins, highlighting the importance of high-throughput two-hybrid screening as a tool to study the complexity of membrane traffic machineries." @default.
- W2079866619 created "2016-06-24" @default.
- W2079866619 creator A5000258094 @default.
- W2079866619 creator A5004863736 @default.
- W2079866619 creator A5052789211 @default.
- W2079866619 date "2015-05-01" @default.
- W2079866619 modified "2023-09-23" @default.
- W2079866619 title "Two-hybrid-based systems: Powerful tools for investigation of membrane traffic machineries" @default.
- W2079866619 cites W1498549914 @default.
- W2079866619 cites W1524710988 @default.
- W2079866619 cites W1559083131 @default.
- W2079866619 cites W1565697070 @default.
- W2079866619 cites W1579879095 @default.
- W2079866619 cites W1884315263 @default.
- W2079866619 cites W1965171930 @default.
- W2079866619 cites W1966828940 @default.
- W2079866619 cites W1967393597 @default.
- W2079866619 cites W1971227936 @default.
- W2079866619 cites W1971598818 @default.
- W2079866619 cites W1971648232 @default.
- W2079866619 cites W1974485263 @default.
- W2079866619 cites W1975230069 @default.
- W2079866619 cites W1976209715 @default.
- W2079866619 cites W1977186059 @default.
- W2079866619 cites W1981146490 @default.
- W2079866619 cites W1982137523 @default.
- W2079866619 cites W1983621817 @default.
- W2079866619 cites W1985425337 @default.
- W2079866619 cites W1985963795 @default.
- W2079866619 cites W1988769775 @default.
- W2079866619 cites W1990425617 @default.
- W2079866619 cites W1992216985 @default.
- W2079866619 cites W1994668150 @default.
- W2079866619 cites W1994993139 @default.
- W2079866619 cites W1997965096 @default.
- W2079866619 cites W1998082866 @default.
- W2079866619 cites W2000596036 @default.
- W2079866619 cites W2001222839 @default.
- W2079866619 cites W2002778633 @default.
- W2079866619 cites W2003445673 @default.
- W2079866619 cites W2004406773 @default.
- W2079866619 cites W2004708626 @default.
- W2079866619 cites W2005178301 @default.
- W2079866619 cites W2005223383 @default.
- W2079866619 cites W2011063992 @default.
- W2079866619 cites W2011261569 @default.
- W2079866619 cites W2012560446 @default.
- W2079866619 cites W2012771354 @default.
- W2079866619 cites W2013699930 @default.
- W2079866619 cites W2013741637 @default.
- W2079866619 cites W2015045770 @default.
- W2079866619 cites W2015608994 @default.
- W2079866619 cites W2017303274 @default.
- W2079866619 cites W2017738734 @default.
- W2079866619 cites W2018619092 @default.
- W2079866619 cites W2021521386 @default.
- W2079866619 cites W2024103356 @default.
- W2079866619 cites W2025871861 @default.
- W2079866619 cites W2028307218 @default.
- W2079866619 cites W2029504852 @default.
- W2079866619 cites W2034499520 @default.
- W2079866619 cites W2036574218 @default.
- W2079866619 cites W2037228773 @default.
- W2079866619 cites W2038867755 @default.
- W2079866619 cites W2041865938 @default.
- W2079866619 cites W2041914510 @default.
- W2079866619 cites W2041957261 @default.
- W2079866619 cites W2045466386 @default.
- W2079866619 cites W2047730219 @default.
- W2079866619 cites W2050305509 @default.
- W2079866619 cites W2052093902 @default.
- W2079866619 cites W2052108496 @default.
- W2079866619 cites W2053282493 @default.
- W2079866619 cites W2053609834 @default.
- W2079866619 cites W2054172569 @default.
- W2079866619 cites W2057311550 @default.
- W2079866619 cites W2057686161 @default.
- W2079866619 cites W2058477407 @default.
- W2079866619 cites W2059418306 @default.
- W2079866619 cites W2063385539 @default.
- W2079866619 cites W2066626586 @default.
- W2079866619 cites W2067305633 @default.
- W2079866619 cites W2067476911 @default.
- W2079866619 cites W2069077656 @default.
- W2079866619 cites W2070360032 @default.
- W2079866619 cites W2070375075 @default.
- W2079866619 cites W2071502814 @default.
- W2079866619 cites W2071993841 @default.
- W2079866619 cites W2073170519 @default.
- W2079866619 cites W2074652755 @default.
- W2079866619 cites W2078313196 @default.
- W2079866619 cites W2079426683 @default.
- W2079866619 cites W2080738247 @default.
- W2079866619 cites W2080764609 @default.
- W2079866619 cites W2080874961 @default.
- W2079866619 cites W2084693864 @default.
- W2079866619 cites W2086053245 @default.
- W2079866619 cites W2086698861 @default.