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• W2079871749 abstract "Objective: Transforming growth factor beta (TGF-) plays a key role in orchestrating wound repair in both the epithelium and mesothelium. Its three isoforms, TGF-1, TGF-2, and TGF-3, have direct roles in regulating the deposition and turnover of extracellular matrix components, vascular in-growth, and fibroblast activation. Previous epidermal studies in murine and human models have shown that an increased TGF-1/2 ratio in healing tissue is associated with increased fibrosis and scarring. Prior peritoneal studies at our institution have demonstrated elevated TGF-1/2 ratios in the range of 2.01–2.34 in surgically abraded peritoneum, contrasting to lower TGF-1/2 ratios of 0.97–1.04 in normal, fresh peritoneum from non-operated animals. Our objective was to determine whether the TGF-1/2 mRNA ratio of surgically abraded but normally healed peritoneum is concordant with that of uninjured peritoneum or peritoneal adhesions.Design: Prospective, comparative, experimental study.Materials/Methods: Cecal abrasion using a previously validated, standardized method was performed on 120 Sprague-Dawley rats, followed seven days later by necropsy. Cecum was harvested from five rats where abrasion had been performed, but healed normally without adhesion formation. Total RNA was extracted from these tissues. Real-time polymerase chain reaction (PCR) using intercalating SYBR Green I dye and rat-specific TGF-1 and 2 primers, with -actin as the normalizing control, was utilized to quantitate the relative changes in TGF-1 and 2 mRNA levels in the tissues.Results: The normalized mean ratios and standard deviations for the five samples were as follows: 1,0.655 ± 0.092, 2,0.611 ± 0.070. The TGF-1/2 ratio derived from these means was 1.072, consistent with that seen in fresh, normal peritoneum from non-operated animals.Conclusions: The TGF-1/2 mRNA ratio from normally healed peritoneum seven days after surgical abrasion maintains the same TGF-1/2 ratio seen in fresh, non-operated tissue. The ratio does not elevate, as is seen in adhesions resulting from surgical abrasion. Manipulation of TGF-1 and 2 isoforms during the early postoperative period to maintain the ratio of non-operated tissues may be a potential therapy to reduce postoperative adhesion formation.Supported by: None. Objective: Transforming growth factor beta (TGF-) plays a key role in orchestrating wound repair in both the epithelium and mesothelium. Its three isoforms, TGF-1, TGF-2, and TGF-3, have direct roles in regulating the deposition and turnover of extracellular matrix components, vascular in-growth, and fibroblast activation. Previous epidermal studies in murine and human models have shown that an increased TGF-1/2 ratio in healing tissue is associated with increased fibrosis and scarring. Prior peritoneal studies at our institution have demonstrated elevated TGF-1/2 ratios in the range of 2.01–2.34 in surgically abraded peritoneum, contrasting to lower TGF-1/2 ratios of 0.97–1.04 in normal, fresh peritoneum from non-operated animals. Our objective was to determine whether the TGF-1/2 mRNA ratio of surgically abraded but normally healed peritoneum is concordant with that of uninjured peritoneum or peritoneal adhesions. Design: Prospective, comparative, experimental study. Materials/Methods: Cecal abrasion using a previously validated, standardized method was performed on 120 Sprague-Dawley rats, followed seven days later by necropsy. Cecum was harvested from five rats where abrasion had been performed, but healed normally without adhesion formation. Total RNA was extracted from these tissues. Real-time polymerase chain reaction (PCR) using intercalating SYBR Green I dye and rat-specific TGF-1 and 2 primers, with -actin as the normalizing control, was utilized to quantitate the relative changes in TGF-1 and 2 mRNA levels in the tissues. Results: The normalized mean ratios and standard deviations for the five samples were as follows: 1,0.655 ± 0.092, 2,0.611 ± 0.070. The TGF-1/2 ratio derived from these means was 1.072, consistent with that seen in fresh, normal peritoneum from non-operated animals. Conclusions: The TGF-1/2 mRNA ratio from normally healed peritoneum seven days after surgical abrasion maintains the same TGF-1/2 ratio seen in fresh, non-operated tissue. The ratio does not elevate, as is seen in adhesions resulting from surgical abrasion. Manipulation of TGF-1 and 2 isoforms during the early postoperative period to maintain the ratio of non-operated tissues may be a potential therapy to reduce postoperative adhesion formation. Supported by: None." @default.
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• W2079871749 date "2002-09-01" @default.
• W2079871749 modified "2023-09-30" @default.
• W2079871749 title "TGF-Beta 1/Beta 2 mRNA ratio in normally healed peritoneum same as in uninjured tissue" @default.
• W2079871749 doi "https://doi.org/10.1016/s0015-0282(02)03674-9" @default.
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