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- W2079874891 abstract "The reddish pigments of red onions (Allium cepa) and flowers of the tulip cultivar Los Angelos, including fragile anthocyanins with aliphatic acylation, were separated in semi-preparative scale by high speed countercurrent chromatography, HSCCC. The lower, polar phase of the biphasic solvent, t-butyl-methylether:n-butanol: acetonitrile:water (2:2:1:5 v/v) acidified with 0.01% trifluoroacetic acid, was used as mobile phase. All the five major anthocyanins in red onion, which contained the same aglycone (cyanidin), were separated from a 750 mg anthocyanin-enriched isolate. Relative to cyanidin 3-glucoside, analogous anthocyanins with additional acyl group(s) (malonyl) and sugar (glucosyl) entailed higher and lower elution volume, respectively. The major pigments in a 430 mg anthocyanin-enriched sample from flowers of the tulip “Los Angelos” were determined to be the 3-O-(6″-rhamnosylglucosides) of delphinidin, cyaniding, and pelargonidin, and the 3-O-[6″-(2‴-acetylrhamnosyl)glucosides] of cyanidin and pelargonidin. Acetylated anthocyanins were eluted after their non-acetylated analogues. Within each of the two groups, acetylated and non-acetylated, individual anthocyanins were separated according to their number of hydroxy-groups on the aglycone B-rings; highest number entailed lowest elution volume. All the acylated anthocyanins in the anthocyaninenriched isolates of both red onions and tulip “Los Angelos” were nearly baseline separated." @default.
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- W2079874891 date "2001-06-30" @default.
- W2079874891 modified "2023-09-27" @default.
- W2079874891 title "SEPARATION OF ACYLATED ANTHOCYANIN PIGMENTS BY HIGH SPEED CCC" @default.
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- W2079874891 doi "https://doi.org/10.1081/jlc-100104379" @default.
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