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• W2079875492 startingPage "2743" @default.
• W2079875492 abstract "In Brief Study Design. Human intervertebral disc cells were harvested from patients with adolescent idiopathic scoliosis (AIS) and from donors with degenerative disc disease. Anulus fibrosis (AF) was separated from nucleus pulposus (NP), and cells were cultured separately in two different cell culture models. Objectives. To investigate changes in gene expression of human disc cells during in vitro expansion and to determine whether cells from adolescent idiopathic scoliosis donors show different gene expression profiles compared with cells from patients operated for degenerative disc disease. Summary of Background Data. During in vitro expansion, cells undergo a dedifferentiation process, which is characterized by a switch in gene expression. Markers for the differentiation and dedifferentiation status of human disc cells are not yet known. Moreover, it is not known whether changes in the gene expression pattern occur during the degeneration process. Methods. Cells from AF and NP tissues were expanded in monolayer and alginate cultures under controlled and defined conditions. Cells were then harvested, and analysis of phenotype was performed using quantitative real-time polymerase chain reaction (PCR). The mRNA expression of Type I, II, and X collagen, aggrecan, and interleukin-1β in scoliosis and degenerative human intervertebral disc cells was analyzed. Results. The gene expression of Type II and X collagen and of aggrecan significantly decreased for both cell types during monolayer expansion. Reexpression of all genes was observed when cells were cultured in alginate. Additionally, NP cells from degenerative tissues displayed significant lower levels of Type II collagen compared with NP cells from scoliosis donors. Conclusions. These results provide a better understanding of how the phenotype of human healthy anddegenerative disc cells is influenced by in vitro expansion. This may be useful for future tissue engineering purposes. Intervertebral disc cells from 27 donors (5 adolescents with idiopathic scoliosis and 22 patients operated for degenerative disc disease) were cultured in monolayer and in a three-dimensional culture system. Changes in gene expression induced by culture system or by degenerative tissue state were analyzed by quantitative polymerase chain reaction." @default.
• W2079875492 created "2016-06-24" @default.
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• W2079875492 date "2005-12-01" @default.
• W2079875492 modified "2023-10-12" @default.
• W2079875492 title "Human Anulus Fibrosis and Nucleus Pulposus Cells of the Intervertebral Disc" @default.
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• W2079875492 doi "https://doi.org/10.1097/01.brs.0000192204.89160.6d" @default.
• W2079875492 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16371897" @default.
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