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- W2079876663 abstract "Abstract A method for preparing chitinase substrate is described; the substrate reproducibly gives high chitinase activity under suitable conditions. For moulting fluid chitinase, these are: a chitin concentration of at least 2 mg/ml, pH 6.5, and presence of calcium; addition of an inhibitor for neutral protease is helpful. Activity may be measured in minutes, either colourimetrically or by counting acid-soluble product from in vivo labelled chitin. N- Acetylglucosamine is the chief soluble end product. Moulting fluid chitinase appears to form a complex with chitin and calcium which successfully competes with EGTA for calcium; the dissociation constant is therefore at least as low as 10 −7 M. A transient increase in product release of chitinase accompanies the first interaction of chitin, chitinase and calcium ions." @default.
- W2079876663 created "2016-06-24" @default.
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- W2079876663 date "1981-01-01" @default.
- W2079876663 modified "2023-09-27" @default.
- W2079876663 title "Biochemistry of insect differentiation. Requirements for high in vitro moulting fluid chitinase activity" @default.
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- W2079876663 doi "https://doi.org/10.1016/0020-1790(81)90029-9" @default.
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