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- W2079876918 abstract "The direct injection of plasma samples after ultrafiltration into a gas chromatograph using a packed injector liner was investigated. Ropivacaine, a local anaesthetic of the amide type and one of its metabolites (PPX) were used as model compounds in this evaluation. Phosphoric acid was added to the plasma to minimize the protein binding. After ultrafiltration, 50 microl of the sample was directly injected into the chromatographic system. No interfering peaks or damage to the GC or MS system were observed using ultrafiltration as a sample-preparation method. The validation of the method demonstrated good linearity and selectivity. The limits of quantification were 1.1 nM (301 pg/ml) and 1.4 nM (325 pg/ml) for ropivacaine and PPX, respectively. The liner had to be changed after 20 injections." @default.
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- W2079876918 date "2001-05-01" @default.
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- W2079876918 title "Direct injection of human plasma samples after ultrafiltration into programmed temperature vaporiser-gas chromatography–mass spectrometry with packed liner" @default.
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- W2079876918 doi "https://doi.org/10.1016/s0378-4347(01)00116-5" @default.
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