Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079877530> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2079877530 endingPage "1811" @default.
- W2079877530 startingPage "1808" @default.
- W2079877530 abstract "An easy and rapid procedure for the determination of glutamine in synthetic peptides and isolated proteins is developed. It involves a prehydrolysis reaction of glutamine residues with bis(1,1-trifluoroacetoxy)iodobenzene (BTI), protein hydrolysis using a microwave technique, and high-performance liquid chromatography (HPLC) after precolumn derivatization of amino acids with dansyl chloride. Optimum conditions for the BTI-mediated conversion of glutamine to the corresponding diaminobutyric acid (DABA) were established. DABA and the proteic amino acids could be simultaneously measured with high sensitivity (2.0 pmol/injection; S/N = 3:1) and good reproducibility (CV 2.8%). The linearity between DABA formation and glutamine concentration was excellent (r = 0.9996). Recovery of DABA (mean recovery 93 ± 4%) from the selected test peptides and proteins (cathepsin G peptide, whey proteins, ovalbumin) was in good agreement with data derived from sequence analyses. Keywords: Glutamine; content in protein/peptides; analysis; BTI; microwave hydrolysis; HPLC" @default.
- W2079877530 created "2016-06-24" @default.
- W2079877530 creator A5045468681 @default.
- W2079877530 creator A5057653565 @default.
- W2079877530 creator A5072989515 @default.
- W2079877530 date "1996-01-01" @default.
- W2079877530 modified "2023-10-17" @default.
- W2079877530 title "Quantitative Analyses of Glutamine in Peptides and Proteins" @default.
- W2079877530 cites W1991494105 @default.
- W2079877530 cites W1993339103 @default.
- W2079877530 cites W2028469023 @default.
- W2079877530 cites W2053860923 @default.
- W2079877530 cites W2073557231 @default.
- W2079877530 cites W2088307742 @default.
- W2079877530 cites W2111784969 @default.
- W2079877530 cites W2178457981 @default.
- W2079877530 cites W2951853895 @default.
- W2079877530 cites W4240388280 @default.
- W2079877530 cites W4245641406 @default.
- W2079877530 doi "https://doi.org/10.1021/jf950511i" @default.
- W2079877530 hasPublicationYear "1996" @default.
- W2079877530 type Work @default.
- W2079877530 sameAs 2079877530 @default.
- W2079877530 citedByCount "24" @default.
- W2079877530 countsByYear W20798775302012 @default.
- W2079877530 countsByYear W20798775302014 @default.
- W2079877530 countsByYear W20798775302015 @default.
- W2079877530 countsByYear W20798775302018 @default.
- W2079877530 countsByYear W20798775302020 @default.
- W2079877530 countsByYear W20798775302021 @default.
- W2079877530 countsByYear W20798775302022 @default.
- W2079877530 crossrefType "journal-article" @default.
- W2079877530 hasAuthorship W2079877530A5045468681 @default.
- W2079877530 hasAuthorship W2079877530A5057653565 @default.
- W2079877530 hasAuthorship W2079877530A5072989515 @default.
- W2079877530 hasConcept C179998833 @default.
- W2079877530 hasConcept C181199279 @default.
- W2079877530 hasConcept C185592680 @default.
- W2079877530 hasConcept C198118469 @default.
- W2079877530 hasConcept C2776923573 @default.
- W2079877530 hasConcept C2779281246 @default.
- W2079877530 hasConcept C2779349466 @default.
- W2079877530 hasConcept C2780626841 @default.
- W2079877530 hasConcept C2780784506 @default.
- W2079877530 hasConcept C28800991 @default.
- W2079877530 hasConcept C43617362 @default.
- W2079877530 hasConcept C515207424 @default.
- W2079877530 hasConcept C55493867 @default.
- W2079877530 hasConcept C94412978 @default.
- W2079877530 hasConceptScore W2079877530C179998833 @default.
- W2079877530 hasConceptScore W2079877530C181199279 @default.
- W2079877530 hasConceptScore W2079877530C185592680 @default.
- W2079877530 hasConceptScore W2079877530C198118469 @default.
- W2079877530 hasConceptScore W2079877530C2776923573 @default.
- W2079877530 hasConceptScore W2079877530C2779281246 @default.
- W2079877530 hasConceptScore W2079877530C2779349466 @default.
- W2079877530 hasConceptScore W2079877530C2780626841 @default.
- W2079877530 hasConceptScore W2079877530C2780784506 @default.
- W2079877530 hasConceptScore W2079877530C28800991 @default.
- W2079877530 hasConceptScore W2079877530C43617362 @default.
- W2079877530 hasConceptScore W2079877530C515207424 @default.
- W2079877530 hasConceptScore W2079877530C55493867 @default.
- W2079877530 hasConceptScore W2079877530C94412978 @default.
- W2079877530 hasIssue "7" @default.
- W2079877530 hasLocation W20798775301 @default.
- W2079877530 hasOpenAccess W2079877530 @default.
- W2079877530 hasPrimaryLocation W20798775301 @default.
- W2079877530 hasRelatedWork W2081478254 @default.
- W2079877530 hasRelatedWork W2168408094 @default.
- W2079877530 hasRelatedWork W2317943501 @default.
- W2079877530 hasRelatedWork W2606142292 @default.
- W2079877530 hasRelatedWork W2806647571 @default.
- W2079877530 hasRelatedWork W2899901573 @default.
- W2079877530 hasRelatedWork W2905734066 @default.
- W2079877530 hasRelatedWork W3041137033 @default.
- W2079877530 hasRelatedWork W4360863538 @default.
- W2079877530 hasRelatedWork W2134450822 @default.
- W2079877530 hasVolume "44" @default.
- W2079877530 isParatext "false" @default.
- W2079877530 isRetracted "false" @default.
- W2079877530 magId "2079877530" @default.
- W2079877530 workType "article" @default.