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• W2079883633 abstract "An automatic method for the column chromatographic separation of lipid mixtures, particularly phospholipids, utilizing convace gradient elution is described in detail. The gradient in this system is produced by an automatic pumping system using accurate controlled volume pumps and variable pumping rates. A discussion of the theoretical aspects of gradient elution in relation to nonlinear absorption isotherms is included, and several chromatographic runs using the system are presented. The system is capable of excellent resolution of naturally occurring phospholipid mixtures and is quite reproducible. Considerable flexibility is available in shape of the gradient produced by the pumping system allowing modification for particular tissue extracts containing phospholipids in varying ratios. The system is completely automatic and, once a run is started, operates untended until completion." @default.
• W2079883633 created "2016-06-24" @default.
• W2079883633 creator A5063036080 @default.
• W2079883633 date "1963-02-01" @default.
• W2079883633 modified "2023-10-13" @default.
• W2079883633 title "An apparatus for production of concave gradients and its application to chromatography of lipids" @default.
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• W2079883633 doi "https://doi.org/10.1016/0003-2697(63)90018-6" @default.
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