FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079886123> ?p ?o ?g. }

• W2079886123 endingPage "293" @default.
• W2079886123 startingPage "277" @default.
• W2079886123 abstract "Mitochondrial ferritin is a recently identified protein precursor encoded by an intronless gene. It is specifically taken up by the mitochondria and processed to a mature protein that assembles into functional ferritin shells. The full mature recombinant protein and its S144A mutant were produced to study structural and functional properties. They yielded high quality crystals from Mg(II) solutions which diffracted up to 1.38 Å resolution. The 3D structures of the two proteins resulted very similar to that of human H-ferritin, to which they have high level of sequence identity (∼80%). Metal-binding sites were identified in the native crystals and in those soaked in Mn(II) and Zn(II) solutions. The ferroxidase center binds binuclear iron at the sites A and B, and the structures showed that the A site was always fully occupied by Mg(II), Mn(II) or Zn(II), while the occupancy of the B site was variable. In addition, distinct Mg(II) and Zn(II)-binding sites were found in the 3-fold axes to block the hydrophilic channels. Other metal-binding sites, never observed before in H-ferritin, were found on the cavity surface near the ferroxidase center and near the 4-fold axes. Mitochondrial ferritin showed biochemical properties remarkably similar to those of human H-ferritin, except for the difficulty in renaturing to yield ferritin shells and for a reduced (∼41%) rate in ferroxidase activity. This was partially rescued by the substitution of the bulkier Ser144 with Ala, which occurs in H-ferritin. The residue is exposed on a channel that connects the ferroxidase center with the cavity. The finding that the mutation increased both catalytic activity and the occupancy of the B site demonstrated that the channel is functionally important. In conclusion, the present data define the structure of human mitochondrial ferritin and provide new data on the iron pathways within the H-type ferritin shell." @default.
• W2079886123 created "2016-06-24" @default.
• W2079886123 creator A5001560379 @default.
• W2079886123 creator A5032747366 @default.
• W2079886123 creator A5038410313 @default.
• W2079886123 creator A5050840680 @default.
• W2079886123 creator A5051403731 @default.
• W2079886123 creator A5076970032 @default.
• W2079886123 creator A5081248464 @default.
• W2079886123 creator A5085669368 @default.
• W2079886123 date "2004-07-01" @default.
• W2079886123 modified "2023-10-16" @default.
• W2079886123 title "Crystal Structure and Biochemical Properties of the Human Mitochondrial Ferritin and its Mutant Ser144Ala" @default.
• W2079886123 cites W1556929642 @default.
• W2079886123 cites W1559146329 @default.
• W2079886123 cites W1571036104 @default.
• W2079886123 cites W1860801498 @default.
• W2079886123 cites W197437642 @default.
• W2079886123 cites W1975197163 @default.
• W2079886123 cites W1978096114 @default.
• W2079886123 cites W1980964234 @default.
• W2079886123 cites W1983419175 @default.
• W2079886123 cites W1986191025 @default.
• W2079886123 cites W1995017064 @default.
• W2079886123 cites W1999088512 @default.
• W2079886123 cites W1999590520 @default.
• W2079886123 cites W2000381161 @default.
• W2079886123 cites W2001641653 @default.
• W2079886123 cites W2004381662 @default.
• W2079886123 cites W2006383772 @default.
• W2079886123 cites W2014179433 @default.
• W2079886123 cites W2016255772 @default.
• W2079886123 cites W2043351987 @default.
• W2079886123 cites W2053769296 @default.
• W2079886123 cites W2055989425 @default.
• W2079886123 cites W2069044991 @default.
• W2079886123 cites W2070019521 @default.
• W2079886123 cites W2085189462 @default.
• W2079886123 cites W2086567353 @default.
• W2079886123 cites W2090148249 @default.
• W2079886123 cites W2096302445 @default.
• W2079886123 cites W2106315897 @default.
• W2079886123 cites W2108816504 @default.
• W2079886123 cites W2115276017 @default.
• W2079886123 cites W2133385897 @default.
• W2079886123 cites W2143305669 @default.
• W2079886123 cites W2150246687 @default.
• W2079886123 cites W2164181570 @default.
• W2079886123 cites W2165011009 @default.
• W2079886123 cites W2248193020 @default.
• W2079886123 cites W24825330 @default.
• W2079886123 cites W274005068 @default.
• W2079886123 cites W4210291672 @default.
• W2079886123 doi "https://doi.org/10.1016/j.jmb.2004.04.036" @default.
• W2079886123 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15201052" @default.
• W2079886123 hasPublicationYear "2004" @default.
• W2079886123 type Work @default.
• W2079886123 sameAs 2079886123 @default.
• W2079886123 citedByCount "106" @default.
• W2079886123 countsByYear W20798861232012 @default.
• W2079886123 countsByYear W20798861232013 @default.
• W2079886123 countsByYear W20798861232014 @default.
• W2079886123 countsByYear W20798861232015 @default.
• W2079886123 countsByYear W20798861232016 @default.
• W2079886123 countsByYear W20798861232017 @default.
• W2079886123 countsByYear W20798861232018 @default.
• W2079886123 countsByYear W20798861232019 @default.
• W2079886123 countsByYear W20798861232020 @default.
• W2079886123 countsByYear W20798861232021 @default.
• W2079886123 countsByYear W20798861232022 @default.
• W2079886123 crossrefType "journal-article" @default.
• W2079886123 hasAuthorship W2079886123A5001560379 @default.
• W2079886123 hasAuthorship W2079886123A5032747366 @default.
• W2079886123 hasAuthorship W2079886123A5038410313 @default.
• W2079886123 hasAuthorship W2079886123A5050840680 @default.
• W2079886123 hasAuthorship W2079886123A5051403731 @default.
• W2079886123 hasAuthorship W2079886123A5076970032 @default.
• W2079886123 hasAuthorship W2079886123A5081248464 @default.
• W2079886123 hasAuthorship W2079886123A5085669368 @default.
• W2079886123 hasConcept C104317684 @default.
• W2079886123 hasConcept C107824862 @default.
• W2079886123 hasConcept C143065580 @default.
• W2079886123 hasConcept C178790620 @default.
• W2079886123 hasConcept C185592680 @default.
• W2079886123 hasConcept C2778319317 @default.
• W2079886123 hasConcept C28452662 @default.
• W2079886123 hasConcept C47701112 @default.
• W2079886123 hasConcept C544153396 @default.
• W2079886123 hasConcept C55493867 @default.
• W2079886123 hasConcept C8010536 @default.
• W2079886123 hasConceptScore W2079886123C104317684 @default.
• W2079886123 hasConceptScore W2079886123C107824862 @default.
• W2079886123 hasConceptScore W2079886123C143065580 @default.
• W2079886123 hasConceptScore W2079886123C178790620 @default.
• W2079886123 hasConceptScore W2079886123C185592680 @default.
• W2079886123 hasConceptScore W2079886123C2778319317 @default.
• W2079886123 hasConceptScore W2079886123C28452662 @default.
• W2079886123 hasConceptScore W2079886123C47701112 @default.

• next