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- W2079894939 abstract "Unlike other retroviruses, human immunodeficiency virus type-1 (HIV-1) can infect terminally differentiated cells, due to the ability of its pre-integration complex (PIC) to translocate via the host nuclear pore complex (NPC). The PIC Nuclear import has been suggested to be mediated by the viral integrase protein (IN), via either the importin α or transportin 3 (TNPO3/transportin-SR2) pathways.We show that in virus-infected cells, IN interacts with both importin α and TNPO3, simultaneously or separately, suggesting a multiple use of nuclear import pathways. Disruption of either the IN-importin α or IN-TNPO3 complexes in virus-infected cells by specific cell-permeable-peptides resulted in inhibition of IN and viral cDNA nuclear import. Here we show that peptides which disrupt either one of these complexes block virus infection, indicating involvement of both pathways in efficient viral replication. Formation of IN-importin α and IN-TNPO3 complexes has also been observed in IN-transfected cultured cells. Using specific peptides, we demonstrate that in transfected cells but not in virus infected cells the importin α pathway overrides that of TNPO3. The IN-importin α and IN-TNPO3 complexes were not observed in virus-infected Rev-expressing cells, indicating the Rev protein's ability to disrupt both complexes.Our work suggests that IN nuclear import requires the involvement of both importin α and TNPO3. The ability to inhibit nuclear import of the IN-DNA complex and consequently, virus infection by peptides that interrupt IN's interaction with either importin α or TNPO3 indicates that for efficient infection, nuclear import of IN should be mediated by both nuclear-import receptors." @default.
- W2079894939 created "2016-06-24" @default.
- W2079894939 creator A5048157747 @default.
- W2079894939 creator A5048335465 @default.
- W2079894939 creator A5068857098 @default.
- W2079894939 creator A5081058474 @default.
- W2079894939 date "2010-09-01" @default.
- W2079894939 modified "2023-09-28" @default.
- W2079894939 title "Transportin 3 and importin α are required for effective nuclear import of HIV-1 integrase in virus-infected cells" @default.
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- W2079894939 doi "https://doi.org/10.4161/nucl.1.5.12903" @default.
- W2079894939 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3037538" @default.
- W2079894939 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21326825" @default.
- W2079894939 hasPublicationYear "2010" @default.
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