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- W2079896701 abstract "It had previously been shown that the number of neurons was greatly enhanced when the culture medium contained an increased K+ and a concomitant decreased Na+ concentration in order to maintain the osmotic pressure. The purpose of the present study was to ascertain if this enhancement was due to the increase in K+ or to the decrease in Na+ concentration. Dissociated ganglia from chick embryos were cultured in a control medium containing normal K+ (6.8 mm) and normal Na+ (140mm) and in medium containing high K+ (40.8 mm) and decreased Na+ (90 mm). Also, keeping the osmotic pressure constant by adding a variety of substances, the dissociated ganglia were grown in media containing normal K+ (6.8 mm) but with reduced Na+ (90 mm). The results showed the following: (a) Low Na+per se is not responsible for the enhancement of number of neurons in media with high K+ and low Na+. (b) The number of neurons is almost the same in media with low Na+ as in media with normal Na+. (c) Elevated choline can enhance the number of neurons but to a lesser extent than K+ can." @default.
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- W2079896701 date "1971-05-01" @default.
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- W2079896701 title "Effect of choline, high potassium, and low sodium on the number of neurons in cultures of dissociated chick ganglia" @default.
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- W2079896701 doi "https://doi.org/10.1016/0014-4886(71)90186-5" @default.
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