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• W2079944549 abstract "Using transposon mutagenesis, we isolated a lipopolysaccharide (LPS) O side-chain (O antigen) mutant strain of Edwardsiella ictaluri. Failure of the mutant to express O side chains was confirmed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) with silver staining and on immunoblots. In contrast, an attenuated strain developed by in vitro subculture did not express changes in LPS that were detectable by SDS–PAGE or immunoblotting. Both the O side-chain mutant and the in vitro subcultured strain were highly attenuated in channel catfish Ictalurus punctatus fingerlings compared with the parent wild-type strain. We conclude that LPS O side chains are an essential virulence factor for E. ictaluri and that loss of virulence in the in vitro subcultured strain was not associated with LPS alterations." @default.
• W2079944549 created "2016-06-24" @default.
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• W2079944549 creator A5052401039 @default.
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• W2079944549 date "2001-12-01" @default.
• W2079944549 modified "2023-10-12" @default.
• W2079944549 title "Phenotype and Virulence of a Transposon-Derived Lipopolysaccharide O Side-Chain Mutant Strain of<i>Edwardsiella ictaluri</i>" @default.
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• W2079944549 doi "https://doi.org/10.1577/1548-8667(2001)013<0291:pavoat>2.0.co;2" @default.
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